原核的

16S rRNA plays an important role in the functional construction of ribosomes and is considered to be a conserved phylogenic marker of prokaryotic evolution.

16S rRNA对于构建功能性核糖体是十分重要的，人们普遍将其作为原核生物进化中保守的系统发育标志。

The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段，采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞，经G418筛选培养基筛选及PCR检测，最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

We compared the effects of ICSI with and without a combination of DTT and EA on male pronuclear formation and embryonic development of porcine oocytes.

比较DTT与电激活的联合处理或单一处理对ICSI后猪卵母细胞雄原核形成与胚发育的影响。

Furthermore, with only a half -dose of DNA in the current system, by the horizontal system under×100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外，利用该系统还可在低倍镜下×100）将DNA常规注射量减半注射，提高了原核胚注射后的成活率，其注射胚的外源基因整合率亦明显提高。

The fertilized egg at the pronuclear stage (prior to the first cell division) is then placed into the fallopian tube hoping that the tubal environment, just as in GIFT, will aid in embryo development, growth and pregnancy.

将原核期的受精卵移植回输卵管环境，此时就同GIFT一样了，这将有助于胚胎的发育。生长和妊娠。

No embryos developed to term after transfer of the pronuclei stage embryos to oviducts of pseudopregnant mice.

将体外受精得到的重组卵来源的双原核胚胎进行输卵管移植，没有胚胎发育到期。

In recent years, both HeV and NiV have continued to emerge and reemerge, The epidemic investigation suggested that the virus could be widely present in the Flying foxes, especially Pteropus lylei in Tailand and Cambodia, which are close to China, moreover, the similar enviorment and Flying foxes species、distribution in the south-east area poese a great threat to pig industry and public health of China, based on the above issues, there is a pressing need for diagnostic technologies on the two virus.

为有效防治亨得拉病毒和尼帕病毒在我国的发生和流行，本研究首先利用原核表达系统成功地表达HeV N、NiV N基因以及NiV G一段主要抗原区基因GC，并证实了两种N蛋白的主要抗原区位于C末端约200aa区域。

Prokaryotic expression vector pET28b㈩/ TprK has been constructed successfully, and pET28b㈩/ TprK protein expressed to have set foundation for preparing prophylactic vaccine and developing serologically diagnostic kit for TP infection.

成功构建人pET28b㈩/TprK原核表达载体，并能高效表达TprK蛋白，为梅毒的预防性疫苗的制备以及进一步研究其血清学诊断试剂奠定了基础。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Accordingly, eukaryotic microorganism cells would die. In the experiment, Staphylococcus aureus was employed as the representative of prokaryotic microorganisms, and Candida albicans and Trichophyton mentagrophytes as the representative of eukaryotic microorganisms, respectively.

在实验中，作为原核微生物的代表使用金黄色葡萄球菌，作为真核微生物的代表使用了白色念珠菌和须癣毛癣菌，并对各种类进行了检测对比。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失