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In comparison with the reported corresponding sequences, the putative amino acid sequence shares high homology with assimilatory nitrate reductase of other microalgae, with identity 78% to Dunaliella tertiolecta, 60% to Volvox carteri, 58% to Chlamydomonas reinhardtii, and 50% to Chlorella vulgaris, respectively.

2.3 杜氏盐藻NR基因原核表达载体在大肠杆菌中的表达和功能鉴定(1)pMAL-NR-2组大肠杆菌SDS-PAGE结果显示:在140kDa左右出现一条特异性条带,分子量与MBP-NR融合蛋白相符(BMP+NR=42.5kDa+99.5kDa=142kDa),在IPTG(0.15mmol/L)37℃诱导时,该蛋白量占全菌蛋白的21.06%;对照组无此特异带。

The present study provides experimental data to understand ADH enzymic property, and to explore the relationship between polymorphism of ADH activity and disease.

为进一步了解ADH的酶学性质,探讨ADH与疾病的关系,本文构建了人ADH原核表达载体,进行了体外表达,并测定了部分酶学参数。

It is a run-of-the-mill prokaryote, an eubacterium, a common inhabitant of the human intestine.

它就是一种普通(run-of-the-mill)的原核生物,一种生活在人类肠道里的真细菌。

This results, combining pathological, evolutionally biological and genetic evidences, suggested it was possible that foreign transposable elements exist widely both in prokaryotes and in eukaryotes.

并且结合许多病理学、进化生物学及其遗传学的证据对异源转座子在原核和真核生物中广泛存在的可能性进行了讨论。

Generative nuclei The two gametic nuclei formed by the division of the generative cell in the pollen tube of angiosperms.

雄原核:在被子植物花粉管中的生殖细胞分裂成两个配子的核。

IL-18-PE38 recombinant immunotoxin expression vector will provide the basis for study on the targeted cytotoxic activity to Th1 cells and may have some potential value in the treatment of Th1 cell-mediated autoimmune diseases.

结论获得了IL-18-PE38融合基因在原核系统的表达,为进一步研究其对Th1细胞的靶向细胞毒性及临床应用奠定了基础。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能;将其编码区序列克隆到原核表达载体pET-30a上,测序鉴定重组质粒,之后进行诱导表达,表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

In order to better understand their phylogenetic relationship and identify the uncertain species, the sequences of the small subunit ribosomal RNA (ssurRNA, 16 S rRNA) genes of nine microsporidia infectious to the silkworm, Bombyx mori, were determined.

微孢子虫是真核生物,但其核糖体及核糖体RNA为原核生物型。为探讨9种家蚕病原性微孢子虫的种属地位及亲缘关系,对已广泛用於生物进化分类的核糖体小亚单位RNA基因进行了研究。

Myxobacteria, which are ubiquitous soil bacteria, are remarkable and unique among prokaryotes in their ability, under conditions of nutrient deficiency, to form fruiting bodies, whereby tens of thousands to millions of cells walk together in a coordinated fashion.

作为一种土壤中广泛存在的细菌,它可以在营养贫瘠的条件下形成子实体,大量细胞共同协作生活,这一特点在原核生物中独一无二。

The therapy groups displayed significant improvements in neuromotor functions and survivals of neuron and oligodendrocyte compared with control groups. These results demonstrated the neuroprotect roles of GGF2, and cationic liposome-mediated GGF2 transgene therapy has potential possibility.

结果:1、大鼠LFP致伤后3—7天,GGF2 mRNA在海马CAl区、CA2区、CA3区、DG及皮层表达明显增加。2、阳离子脂质体介导GGF2转基因治疗可促进大鼠伤后行为学的恢复,可促进损伤区神经元及少突胶质细胞的存活。3、原核表达方法得到GGF2重组蛋白。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。