原核的

Determination of the properties of lactase expressed in E.coli.

原核表达乳糖酶的酶学性质测定制备原核表达乳糖酶的原酶液。

OBJECTIVE: To construct a prokaryotic expression plasmid of the main catalytic domain of dipeptidyl peptidase of murine fibroblast activation protein αand obtain the target fusion protein in order to provide basis for production of antibodies and anti-tumor vaccine targeting FAPα.

目的：构建小鼠成纤维细胞激活蛋白α二肽基肽酶催化核心序列的高效原核表达载体，进行该序列的原核表达、纯化和复性，为下一步高效抗体制作和以FAPα为靶标的抗肿瘤疫苗研发提供实验基础。

Objective To study factors that may increase the expression of human α synuclein gene in prokaryotic cells.

目的 研究影响人α突触核蛋白(α synuclein)基因在原核细胞中表达的常见因素，以提高其在原核细胞中的表达量。

ABSTRACT］ObjectiveTo construct the prokaryotic expression vector of tegument protein pp85 of human herpesvirus 7 and to express it.

摘要］ 目的构建人疱疹病毒7型(HHV7)开放读码框（ORF U14的原核表达载体，并且进行原核表达。

A member of the kingdom Monera, comprising the prokaryotes and including all bacteria.

原核生物无核原生物王国的一员，由原核生物组成，包括所有细菌

Bacteria A kingdom containing all the prokaryotic organisms, formerly called Monera or Prokaryotae.

细菌：包括所有原核生物体的一界生物，以前叫做原核生物界。

The results of protein synthesis of NT embryos indicated that the protein synthesis pattern of NT embryos and fertilized eggs was similar. 19. 2 KD protein existed in 32-cell stage donor embryos disappeared in NT embryos at the pronuclear stage, however, this protein was reprogrammed to reappeared in 32-cell stage NT embryos.

七、核移植胚的蛋白合成分析结果表明，原核期核移植胚与原核期受精卵具有相同的合成模式。32-细胞期供体胚中的19.5KD蛋白在原核期重组胚中消失，而在32-细胞期重组胚中再度出现，发生了蛋白合成的重序过程。

Compared genes up- and down-stream IL-21 between Tetraodon nigroviridi, Fugu rubripes and human, a high degree of conservation of synteny was found.

将TnIL-21 CDS序列克隆到原核表达载体pET32b中，成功地构建重组载体TnIL21-pET32b，并建立其原核表达体系，成功诱导目的蛋白的大量表达。

We spliced the pronuclei vector and microinject the remain DNA sequence to pronuclei of the mouse zygote.We got 29 mice of G0 generation ,and 8 of the 29 mice are positive result which checked by method of PCR.

构建的乳腺特异性表达载体去除原核部分(αS1-casein基因调控序列+人胰岛素原基因)，显微注射小鼠受精卵雄原核，获得29只G_0代小鼠，PCR检测出其中8只为转基因刚性小鼠。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失