原核的

MethodUsing the canis MC4R DNA as template,the specific primers were designed. After PCR amplification,product was cloned into pGEX-4T-1 vector by LP Recco PCR cloning technique,the recombinant pGEX-4T-1-cMC4R was transferred to E.

方法以Beagle犬基因组DNA为模板，经PCR技术扩增目的片段，利用LP Recco PCR克隆技术将目的片段直接重组到原核表达载体pGEX-4T-1上，转化到E.coli DH5α，筛选阳性克隆，BamHI、XhoI酶切鉴定，DNA 测序检测插入序列的正确性。

Suum were reported to be suitable vaccine candidates for the control of Ascaris infection. Here we cloned As37 gene by reverse transcription-polymerase chain reaction using primers based on the pubmed cDNA sequence of As37 gene.

作者已克隆了猪蛔虫rAs37基因并进行了原核表达，并通过对重组融合蛋白As37进行免疫原性分析，显示其具有良好的免疫学活性。

To clone the site A of S gene of transmissible gastroenteritis virus and to construct the prokaryotic expression vectors of site A.

对猪传染性胃肠炎病毒S基因A抗原位点进行克隆和原核表达载体的构建。

Objective To obtain the recombinant protein of an antigen gene Ts88 of Trichinella spiralis and identify the characteristics of the recombinant protein.

目的 原核表达旋毛虫抗原基因Ts88，并对重组蛋白的抗原性进行鉴定。

Antiserums against the non-structural proteins of those viruses were also prepared by prokaryotic expression.

原核表达制备了24个功能蛋白的抗血清。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

The gut of lower termites harbor a complex microbial community,including eukaryotic flagellates and prokaryotic bacteria and archaea,which play important roles in the wood-cellulose digestion of these termites.

低等白蚁肠道里存在着复杂的微生物区系，包括真核微生物鞭毛虫和原核生物，细菌及古细菌。

The CP gene of WVMV-BJ was amplified by RT-PCR, and ligated to the expression vector pET22b.

采用RT-PCR方法自WVMV-BJ的基因组中扩增出其CP基因，连接到原核表达载体pET22b上。

Prokaryotic expressed protein products of candidate cDNAs were purified by SDS-PAGE and used as immunogens to prepare murine antisera.

5利用SDS-PAGE分离原核表达产物，并以纯化的表达产物为免疫原免疫小鼠，制备抗血清。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失