原核的

So, firstly we studied pronuclear embryo of rabbit by sequence culture that is a more developed in vitro culture system.

因此，我们首先对兔原核期胚胎采用一种较先进的体外培养系统——序贯培养进行了研究。

The technique of pronuclear microinjcetion is the most reliable and popular method for transgenic livestock production to date.

到目前为止，原核注射是最可靠，也是使用最广泛的动物转基因方法。

Sperm morphological change after ICSI and factors affecting the pronuclear formation and embryo development of buffalo oocytes after ICSI were investigated in this study.

本研究主要探讨水牛胞质内受精后精子的形态变化、原核形成类型及相关影响因素。

The cleavage and pronuclear formation rate of oocytes treated with 5μmol/L Ion were significantly higher than that of oocytes with EH treatment.

结果发现，5μmol／L Ion激活处理水牛卵母细胞分裂率（63.0％vs 54.2％，P＜0.05）及原核形成率显著高于EH处理的卵母细胞。

Icrofilaments were mainly distributed in the cortex area which chromosomes located at mataphase of second meiosisand concentrated around pronuclei after pronuclear formation.

II 期卵母细胞微丝主要集中在染色体所在的皮层区域，原核形成后微丝绕核重新聚集。

Protozoa is a special and independent kingdom of microorganism in theprocessing of the evolution from prokaryote to eukaryotic cell.

原生动物是区别于原核生物和高等真核生物的一个特定类群。

The vector was linearized and injected into 817 fertilized eggs of mice in which coagulation factor IX gene has been knocked out. The manipulated embryos were transferred into the oviducts of 45 pseudopregnant females, from which 63 offsprings were obtained.

将其线性化后，用显微注射法注射入817只凝血因子Ⅸ基因剔除小鼠受精卵雄原核，再将它们分别回输45只假孕受体母鼠的输卵管中，共产仔69只，存活63只。

Objective To construct an expression vector for fusion gene of heat-liable enterotoxin B subunit and major outer membrane protein of Chlamydia psittaci and express the fusion gene in prokaryotic cells.

目的构建含大肠杆菌热不稳定肠毒素B亚单位和鹦鹉热衣原体主要外膜蛋白融合基因的表达载体，并在原核细胞中表达融合蛋白。

Objective: To construct the prokaryotic expression vector of human cancer-testis gene NY-ESO-1 by the method of LP Recco PCR Cloning.

目的：利用PCR产物靶向克隆法构建人癌－睾丸抗原NY-ESO-1的原核表达载体。

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失