原核的

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

Vitreoscilla hemoglobin is the only well characterized prokaryotic oxygen-binding protein that allows bacterium to grow aerobically even under poor oxygen conditions.

透明颤菌血红蛋白（Vitreoscilla hemoglobin， VHb）是唯一一种研究得较为透彻的原核生物氧结合蛋白血红蛋白。

In prokaryotes the nuclear material is simply a circular strand of DNA, not complexed with histone proteins, and cell division is amitotic.

在原核生物中，核物质仅是一个简单的环状 DNA ，而不与组蛋白结合，并且细胞为无丝分裂。

Our study designed two pairs of specific primers and cloned two molecular weight of 63ku from Schizaphis graminum, and Rhopalosiphum maize utilizing PCR technique and have done the prokaryotic expression, the main results are as follows:(1)Amplified the Buchnera groEL of Schizaphis graminum, and Rhopalosiphum maize Yangling Biotype at first time all over the world ,and sequenced the full lenghth gene.

本研究设计了特异性引物，利用PCR技术从玉米蚜和麦二叉蚜中克隆出蚜虫Buchnera groEL基因并进行原核表达研究，主要结果如下：(1)从杨凌生物型玉米蚜和麦二叉蚜中在国内外首先扩增出Buchnera groEL基因，测定了全长基因序列，并对相关的同源基因核苷酸进行了比较。

Dissimilatory nitrate reduction in fungi includes denitrification and ammonia fermentation, which occurs under the conditions of limited aeration.

真菌异化硝酸盐还原途径的发现打破了反硝化仅存在于原核细胞这一传统观念。

The gene of human proinsulin mutant (A21Asn→Gly,B17Leu→Ile,B31Arg,B32Arg,GIRR ) was synthesized and recombinated to the fusion protein expression vector pTXB1 and expressed in E.coli BL21(DE3) with high protein level.

构建人胰岛素原突变体GIRR（A21Asn→Gly，B17Leu→Ile，B31Arg，B32Arg）基因的原核融合表达载体并进行表达和纯化，进一步对其性质进行研究。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

Plantlike organisms include cyanobacteria in the kingdom Monera.

类似植物体包括在原核生物界的氰细菌。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失