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By combining with the author's own research work, the progresses of study on sperm egg identification, acrosomal reaction, cortical reaction, and formation of male and female pronucleus in decapod crustacean are reviewed in the paper.

结合作者近期的研究工作,从精卵识别、顶体反应、皮层反应、雌雄原核的形成和结合等方面综述了近年来十足类甲壳动物受精细胞学方面的研究成果,并展望了这一领域的研究与应用前景。

Packaged within geminate particles, the genomes of geminiviruses are single-stranded circular DNAs that are replicated mainly by a rolling circle mechanism, characteristic to prokaryotes. Previous researches have indicated that geminiviruses can generate various DNA replication forms in bacteria, suggesting that geminiviruses possess the potential to regulate the expression of certain viral genes in prokaryotic systems.

双生病毒在植物细胞中是主要以原核生物形式的rolling circle replication方式做复制,而先前研究指出双生病毒可以在原核系统里进行复制,因此,双生病毒基因体中应具有在原核系统中亦能表现的调控机制,除了已知的基因外,是否有其他未知的基因参与这项机制仍不清楚。

In the current study, a series of cationic α-helical peptides of 20 a.a. were designed and synthesized based on four structural parameters, including charge, polar angle, hydrophobicity, and hydrophobic moment. Several methods were performed to describe the influence of these parameters on the antimicrobial and hemolytic activity, and on the permeabilizing effect on model membranes. The potential of these parameters to increase the antibacterial activity and to improve the prokaryotic selectivity is assessed.

本实验利用自行设计合成的人工抗菌胜肽当模型,固定长度为 20 个胺基酸,改变其电荷、极性角度、疏水性及疏水性矩等四种结构特性,并利用其对各种不同病原菌之抗菌活性及对兔子红血球细胞的溶血活性来研究其结构特性的改变对其抗菌活性、选择性及微脂体膜的渗透性之影响,以期望改变结构参数能增加抗菌活性及对原核的选择性。

These results indicate that,(1) appropriate concentration of CHX can enhance the activation efficiency of oocytes;(2) CB can increase the two pronuclei formation rate of oocytes by inhibiting the extrusion of second polar body.

这些结果说明:(1)适当浓度的CHX能提高卵母细胞激活效果;(2)CB能抑制卵母细胞激活后第二极体排出,从而提高原核的形成率。

These results indicate that CHX at an appropriate concentration can improve the parthenogenetic development of buffalo oocytes, CB at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei.

结果表明,适当浓度的CHX能提高卵母细胞激活效果,适当浓度的CB可抑制卵母细胞第二极体的排出,提高孤雌激活后双原核的比例。

And the male pronucleus moved to the female pronucleus during the period of associating of the two pronucleus.

并且在系列的样品中可观察到,在雌雄原核联合的过程中具有雄性原核向雌性原核移动的趋势。

The results show that FISH can be used in experimental study and clinical application of PGD; 3. The cause of multi- pronucleus in embryos from IVF or ICSI would be different.

IVF受精和 ICSI受精的3PN胚胎发育有差异,证明*F受精和 ICSI受精的3PN中的原核的来源是不同,导致胚胎第一次卵裂的方式也不同。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P<0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P<0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB,可明显提高双原核的形成率(38.9%vs 15.1%,P<0.05)和桑椹胚/囊胚发育率(40.7%vs 26.8%,P<0.05),但对其激活分裂率和总原核形成率无明显影响(P>0.05)。

Even though the older oocytes were easier to activate and developoed more rapidly to the pronuclear stage than younger ones after artificial stimulation, the percentage of blastocyst development was lower than that in younger oocytes.

大龄卵比小龄卵易于激活而且形成原核的速度比小龄卵快,但卵激活后发育至囊胚的比例较小龄卵低。

To pronuclear formation rate after oocyte in vitro maturation 18 hours and parthenogenticactivation, oocytes derived from SM is significantly higher than oocytes from PM,oocytes derived from IM is significantly lower that of oocytes detrived from PM and SM.

对于孤雌刺激反应而言,无论是何种类型的卵母细胞成熟18小时后,自发成熟卵母细胞激活后形成原核的比率高于被动成熟的卵母细胞,诱导成熟的卵母细胞的原核形成率显著低于自发和/或被动成熟的卵母细胞。

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