原核生物

Objective To construct prokaryotic recombinant plasmids of transcriptional co-activator gene of Clonorchis sinen sis, express and purify the recombinant protein and analyze its biological function.

摘要目的构建华支睾吸虫成虫转录辅激活因子(transcriptionalcoactivator ，TC)基因原核重组质粒，进行原核表达、鉴定及生物功能分析。

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

Bacteria A kingdom containing all the prokaryotic organisms, formerly called Monera or Prokaryotae.

细菌：包括所有原核生物体的一界生物，以前叫做原核生物界。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

A improved screening system for molecular identification of prokaryotes is suggested.

提出了比较系统和完整的原核生物分子鉴定分类的筛选体系。

In prokaryotes the hereditary material is found in the nucleoid.

在原核生物遗传性材料在类核小体被找到。

Meanwhile, many of the groups can be found their syntenic regions in prokaryotes.

许多基因群可以在原核生物中发现共线性区域。

Some of these features probably arose through endosymbiosis of prokaryotes.

其中某些特征可能源于原核生物的内共生。

MRNA in prokaryotes that contains more than one gene to be translated.

含有不止一个需转译基因的原核生物mRNA.cistron =基因。

Many short syntenic regions of HGT genes between rice and prokaryotes suggest that segmental insertion might be a transfer mechanism for HGT in rice.

许多水稻与原核生物间短的水平转移基因共线性区域表明片段插入是水稻HGT的一种转移机制。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。