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The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae, App)鞭毛蛋白编码基因的重组表达质粒,对其编码蛋白进行生物信息学分析,并将其在原核细胞中进行表达。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板,根据其鞭毛区与其它细菌高度同源性设计引物,利用PCR方法扩增鞭毛蛋白基因flic片段,将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定,再将其亚克隆与载体pGEx-kG分别双酶切和连接,构建重组表达载体pGEX-flic,并将其转化大肠杆菌工程菌BL21,进行原核表达。

Mitochondria are self-replicating, and probably they are the evolutionary descendants of what were once free-living prokaryotes.

线粒体可以自我复制,很有可能是曾经游离原核生物演化而来的后代。

Objective To obtain high yield of recombinant human apolipoprotein A-I with normal structural and functional properties by a prokaryotic expression system.

目的 为获得高产量并具有正常结构和生物学活性的原核表达人载脂蛋白A-I。

Overview Glycolysis is a set of reactions that take place in the cytoplasm of prokaryotes and eukaryotes.

概述 糖酵解是发生在原核细胞和真核细胞的胞质中的一组反应。

The haploid nucleus of a ''.

原核授精的核聚变之前的''。

Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1/HN株的己糖激酶编码基因,构建其原核和真核表达重组质粒,测定其序列,比较它及它推导的蛋白质与恶性疟原虫其他株和人之间的差异。

We infer that hira gene is related to sperm nucleus decondensation in fishes.

在果蝇中的研究已经表明hira基因与精核解凝形成雄性原核有关。

In addition, the EO gene of hog cholera lapinized vaccine strain was

同时将兔化弱毒株EO基因分别置于原核与真核表达系中进行了表达,并对重组蛋白进行了初步的免疫学分析。

Hog cholera lapinised virus; E2 gene; Prokaryotic Expression; Indirect ELISA

猪瘟兔化弱毒; E2 基因;原核表达;间接 ELISA

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。