原核

Objective To compare the efficiency of constructing three truncated Echinococcus multilocularis18 gene expression vector using two clone methods.

目的比较3种截短的泡球蚴Em18基因聚合酶链反应扩增产物经TA克隆和直接酶切2种方法，在原核表达质粒构建中的运用。

We insist that the questen will not be a question if the real human derived arresten is obtained, for it is a human body endogenesis fragment of protein.

但是，上述双方均是以原核表达的arresten为研究对象，存在蛋白的翻译后加工及修饰的不完善，从而可能造成其活性改变，这使得这种争论变得含混不清。

Key words: Macaca mulatta;Entamoeba histolytica;hgl gene;LC3 segment;prokaryotic expression

川西猕猴；溶组织内阿米巴；凝集素hgl基因；LC3段；原核表达

Objective To establish a prokaryotic expression system for expressing mouse enterokinase which could recognize and cut the sequence of4-Lys in order to provide a technique platform for application of recombinant enterokinase.

目的：制备能在原核表达系统中表达并特异性识别4-Lys序列的鼠源性肠激酶，为推广应用重组肠激酶提供技术平台。

Recombinant mouse enterokinase can be expressed in Ecoli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

For example, the eukaryotic protein expressed in E.

目前，虽有原核表达OPG的报道，但仍存在一些不足。

What are the most significant differences between the organization and expression of prokaryotic genes and eukaryotic genes?

原核生物的基因与真核生物的基因在组织形式和表达方式方面有哪些主要的区别？

They appear to have a mixture of prokaryotic and eukaryotic genes.

它们被认为是某些真核生物基因和原核生物基因的混合体。

This last idea has not received much acceptance, since flagella lack DNA and do not show ultrastructural similarities to prokaryotes.

后一种想法没有被广泛接受，因为鞭毛缺乏DNA，在结构之外和原核生物也没有明显相似性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐