原核

The expression plasmid pGEX-5X-1/DFF45 was constructed and transformed into E.coli BL21, and the expression of GST-DFF45 protein was induced by IPTG. After purification, the fusion protein GST-DFF45 was used to immunize Oryctolagus cuniculus to obtain the antibody serum.

构建pGEX-5X-1/DFF45原核表达质粒，转化大肠杆菌BL21，用IPTG诱导融合蛋白表达，经纯化后免疫日本大耳白兔得到多克隆抗体并用CNBr-activated Sepharose？

Prokaryotes lack organelles such as nucleoli, mitochondria, plastid s, Golgi apparatus, and do not exhibit cyclosis.

原核生物无细胞器，如核仁、线粒体、质体、高尔基体等，不出现胞质环流。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

By combining with the author's own research work, the progresses of study on sperm egg identification, acrosomal reaction, cortical reaction, and formation of male and female pronucleus in decapod crustacean are reviewed in the paper.

结合作者近期的研究工作，从精卵识别、顶体反应、皮层反应、雌雄原核的形成和结合等方面综述了近年来十足类甲壳动物受精细胞学方面的研究成果，并展望了这一领域的研究与应用前景。

A recombinant protein of Bla g 2 has been obtained, which is soluble in the supernatant and therefore can avoid a process of denaturalization and renaturation of the recombinant.

获得了真核表达的德国小蠊主要变应原重组蛋白Bla g 2，该蛋白以可溶性蛋白的形式分泌到培养液中，避免了原核表达的变复性过程。

All of the traits suggest that dinoflagellate maybe is the intermediate species between prokaryotes and eukaryotes.

寇氏隐甲藻细胞超微结构的特殊性表明，这个物种可能是原核生物向真核生物进化的中间过渡类型。

The results of the experiment show that menadiol sodium diphosphate has no mutation effects on the procaryotic cell in vitro.

结果 当甲萘氢醌二磷酸酯钠的浓度在0.5、5、50、500、5000μg/皿，无论加或不加S9混合物时其对4株沙门菌株的回复突变作用均为阴性。结论甲萘氢醌二磷酸酯钠在体外对原核细胞无诱变作用。

Superoxide Dismutases are ubiquitously expressed antioxidant enzyme in aerobic organisms and catalyze dismutation of superoxide anion to hydrogen and molecular oxygen,the first step in active oxygen-scavenging systems.

超氧化物歧化酶(superoxide dismutase，SOD)在需氧原核生物和真核生物中广泛存在，是活性氧清除系统中第一个发挥作用的抗氧化酶。

And obtained recombinant plasmid PGEM-VP3 by linking prokaryotic expressionby vecto r PGEM-4T-1,and identified by PCR、double restriction enzyme analysis and sequence analysis,the result showed that it's true.

用EcoR I和XhoⅠ双酶切该片段并回收该片段，将此基因片段克隆至相同酶切回收后的PGEM-4T-1原核表达载体中，获得重组质粒PGEM-VP3，经PCR鉴定，限制性内切酶双酶切分析，证实了重组质粒的正确性。

By means of polymerase chain reactionand DNA recombinant techniques,the gene encoding mature ouse IL-4 was obtained from plasmid pCD-mIL4 and was inserted into expressionvector pBV220,which contains _RP_L double promoters,synthesized SD sequence and temperaturesensitive CIts857 gene,thus an expression lone eferred as pBV-mIL4 was constructed.

该质粒含有 P_RP_L 串联启动子，合成 SD 序列和温度敏感的CIts857基因，并且去除了完整小鼠 IL—4 cDNA 中3′侧翼非编码区，以原核系统高频使用的强终止密码子TAA 代替了原小鼠 IL-4基因的终止密码子 TAG，经 SDS-PAGE 及凝胶密度扫描分析，表达的重组小鼠 IL-4分子量约为14.2KDa，占总菌体蛋白量的25～30%。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失