原核

Short time delay dont result in preimplantation embryos death, but impacted blastula rate of pronucleus embryos.

短时间滞留不会引起着床前胚胎死亡，但却使原核胚发育到囊胚能力显著下降。3。

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本实验从抗除草剂转基因Bobwhite小麦中，利用PCR克隆的方法扩增出bar基因全长，并在原核表达系统中表达，鉴定表达蛋白的活性，将能够正确编码PPT乙酰转移酶的bar基因片段，经过适当的修饰构建入真核表达载体。

Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果：单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合，与NIH3T3细胞无反应；1H3与原核表达的c-erbB2胞外区蛋白有特异性反应，而与无关蛋白KDR无反应。

We detected the frozen-thawed sperm of 36 Simmental,23 Charolais and 12 Limousin,the results showed that the frozen-thawed sperm of these three breeds could penetrate the zona-free ova of hamster,and formed male pronucleuses.

对36头西门塔尔、23头夏洛来和12头利木赞牛冷冻精子的实验结果表明：三个品种的牛冻精均能穿入去透明带仓鼠卵，并形成发育良好的雄原核。

We now know that chloramphenicol specifically affects the ribosomes of prokaryotic cells, thus inhibiting protein synthesis.

现在我们知道抗生素对原核生物细胞中的核糖体有特殊效应，从而能抑制其蛋白质的合成。

Because the dimeric form is the active form to exert its biological activities, many trials failed to express TGF β s in prokaryotic systems because of their complex folding manner and disulfide patterns. In 1991 Cerletti and coworkers from Ciba-Geigy Corporation in Switzerland successfully finished the functional expression of human TGF β 2 in bacteria, but the details are protected by the patent.

由于双体是其发挥生物活性的形式，TGF β复杂的折叠方式和二硫键配对使其早期原核基因工程研究都以失败而告终。1991年，瑞士Ciba-Geigy公司的Cerletti等成功完成了人TGFβ2基因的细菌表达，但其技术细节被专利加以保护。

To obtain the fertilized eggs with clear male pronuclei is the key of success .

显微注射法是制备转基因动物的首选方法，而原核清晰受精卵的获得是影响显微注射成败的关键。

As they are evenly dispersed throughout genomes, usually characterized as codominant and highly polymorphic systems amenable to genotyping by polymerase chain reaction, and generally inherited in a Mendelian fashion, microsatellites have become instrumental as genetic markers in many areas.

摘 要：微卫星标记广泛分布于原核生物和真核生物基因组中，具有密度大、多态性丰富、遵循孟德尔分离定律共显性遗传、易于PCR扩增等特点，已成为近年来最引人注目的新型DNA标记。

Study on Cloning the Gene of β-1, 3-glucanase in Tea Plant and Its Expression in Escherichia Coli

论文标题：茶树β-1，3-葡聚糖酶基因的克隆及原核表达分析

A fusion gene of green fluorescent proteinand movement protein of cucumber mosaic viruswas obtained by using recombination-PCR.

采用重组-PCR方法，扩增获得绿色荧光蛋白与黄瓜花叶病毒运动蛋白融合基因，将其克隆到pKEN上，构建了该融合基因的原核表达载体pKENG-M。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失