原核

In addition, these two purified proteins could react with a specific antibody against mouse VEGF as expected. Conclusion Recombinant Xenopus laevis VEGF and its control mouse VEGF protein may provide tools for further study of anti-tumor active immunity with this xenogeneic protein vaccine in mouse tumor models.

重组非洲爪蟾和小鼠血管内皮细胞生长因子的原核表达、分离纯化及鉴定成功，为进一步研究异种VEGF蛋白质疫苗抗小鼠肿瘤模型奠定了基础。

First, nucleic acid sequence encoding ectodomain of AMAl was amplified from Pf genome and, was cloned into prokaryotic expression vector.

首先，自P.f基因组DNA中扩增出编码AMAl胞外域的基因片段，经测序确证后，克隆入原核表达载体。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

The effect of the specific inhibitor cibacron blue of GSTs and antiparasitic drugs albendazole, praziquantel and artesunate on the activities of recombinant GSTM, GST1 and GST2 was evaluated.

研究目的解析三个胞质GSTs的基因结构及其特点，克隆三个胞质GSTs基因，鉴定原核表达的重组GSTs的免疫反应性、免疫原性、催化活性及其免疫定位和抑制剂、常用抗寄生虫药对催化活性的影响。

A high level of expression of GST-NOR1 is obtained in JM 105, and its antiserum can be prepared successfully.

成功获得了高效表达NOR1基因的原核表达产物，通过对该融合蛋白的纯化为它的多抗制备奠定了基础。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

It is possible that the rLL37 is expressed in procaryotic cell by fusion and it possesses a strong bactericidal activity.

改良的人源性LL37多肽以原核细胞进行融合表达是可行的，并具有较强的杀菌活性。

Is expressed in procaryotic cell by fusion and it possesses a strong bactericidal activity.

37多肽以原核细胞进行融合表达是可行的，并具有较强的杀菌活性。

Successful construction and expression of PEA in prokaryotic expression vector are basis for further study of genetic engineering vaccine and recombinant inununotoxin for immune target therapy.

PET-32a-PEA原核表达质粒的成功构建及表达，为进一步研究有效的基因工程疫苗和免疫导向治疗的基因重组毒素奠定了基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失