原核

Key words: Brucella abortus;omp25 gene;cloning;prokaryotic expression

流产布鲁氏菌；omp25基因；克隆；原核表达

Swine;Chlamydophila abortus; omp1 gene;clone; prokaryotic expression

猪；流产嗜性衣原体；omp1基因；克隆；原核表达

Thioredoxins, a ubiquitous heat-stable, acidulous small proteins with a redox activedisulfide bridge in its conserved motif -CPPC-, distribute universally in eukaryote andprokaryote and have a molecular mass of approximately 12Kda.

硫氧还蛋白是一类广泛存于原核生物和真核生物的热稳定、酸性小分子量蛋白质(其分子量约为12Kda)。

Zeocin resistance gene has eukaryote and prokaryote promoters, so it is used to screen by resistance in both mammalian cell lines and bacteria.

Zeocin抗性基因含有真核和原核两个启动子，可以在真核和原核细胞中进行抗性筛选。

Early cleavage rate,embryo quality,the rate of blastocyst,implantation and pregnant rates from these zygotes were observed,and the transferred embryos were selected according to the pronuclei orientation and early cleavage time.

根据受精卵原核构型的不同分为四组，结合构型类型及早期卵裂与否进行选择性移植，通过观察优质胚胎率、囊胚形成率、种植率和怀孕率，来评估合子原核构型和早期卵裂与胚胎发育潜力的关系。

We spliced the pronuclei vector and microinject the remain DNA sequence to pronuclei of the mouse zygote.We got 29 mice of G0 generation ,and 8 of the 29 mice are positive result which checked by method of PCR.

构建的乳腺特异性表达载体去除原核部分(αS1-casein基因调控序列+人胰岛素原基因)，显微注射小鼠受精卵雄原核，获得29只G_0代小鼠，PCR检测出其中8只为转基因刚性小鼠。

There are many documents indicated many kinds of pathogenic bacteriums have the ability to lead latent infection, maybe which is related with the genome conservatived TA system of prokaryote.The TA system is made up of toxin and antitoxin and is concerned to be quality control system of prokaryote.

研究结果提示，很多不同种属的病原菌都具有形成潜在感染的能力，可能与原核生物染色体基因组上保守的TA系统有关，其主要由毒素和抗毒素位点组成，被认为是原核生物基因表达的质量控制系统。

Complicated than procaryotes. These cells, exemplified by the yeast in Figure 4.1-3, have a membrane surrounding the cell which is similar in structure to the procaryote membrane.

真核细胞，具有真正的细胞核，其结构要比原核生物复杂的多，以图4.1-3中所示的酵母菌为例，和原核细胞一样真核细胞也具有一层细胞膜。

FtsZ, a functional protein existed in prokaryotes and plants controlled the division of prokaryotic cells and plastids in plants.

FtsZ是广泛存在于原核生物和植物中的一种功能蛋白，它控制着原核细胞和质体的分裂过程。

In most species they are composed of roughly equal amounts of protein and ribosomal RNA , the ribosome consists of two unequally sized rounded subunits arranged on top of each other like a cottage loaf, eukaryotic cells have larger ribosomes than prokaryotic cells but the ribosomes in mitochondria and chloroplasts are about the same size as prokaryotic ribosomes.

在大多数的物种中，蛋白质的量和 rRNA 量是大体相等的。核糖体包括两个大小不同的亚单位，上部结合形成小屋状结构。真核生物细胞中的核糖体要比原核细胞的核糖体大，但是线粒体和叶绿体中的核糖体与原核细胞的核糖体大小相当。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐