原核

MRNA in prokaryotes that contains more than one gene to be translated.

含有不止一个需转译基因的原核生物mRNA.cistron =基因。

In order to study the expression, subcellular distribution and the function of NS2 protein, the coding region of NS2 was amplified from the hindgut tissue of cockroaches infected with PfDNV by RT-PCR and then the recombinant prokaryotic expression vector pET28a-NS2 was constructed. The recombinant plasmid was transformed into E. coli BL21 (DE3) to express the 6×His fusion protein in the bacteria.

为了对该基因进行深入的功能研究，从感染了黑胸大蠊浓核病毒的蟑螂的后肠组织中通过RT-PCR得到ns2基因编码序列，将其构建于原核表达载体pET-28a，转化大肠杆菌BL21(DE3)获得融合表达产物。

Objective: To study the effects of prokaryotically expressed recombinant human NDRG2 on tumor cell lines.

目的：考察原核表达的重组人NDRG2蛋白对肿瘤细胞的作用。

Many short syntenic regions of HGT genes between rice and prokaryotes suggest that segmental insertion might be a transfer mechanism for HGT in rice.

许多水稻与原核生物间短的水平转移基因共线性区域表明片段插入是水稻HGT的一种转移机制。

The scientists will transfer the pro-nuclei -- the components of a human embryo nucleus -- made by one man and woman into an unfertilised egg from another woman.

科学家们将把取自于一男一女受精卵的原核（pronuclei，人类胚胎细胞核的构成成分）移植到另外一名妇女提供的一个未受精的卵子中去。

Expression of recombinant mature arctic fox interferon gamma in bacterial system was confirmed by SDS-PAGE and Western blotting analysis.

应用原核表达系统高效表达北极狐g-干扰素成熟肽蛋白， SDS-PAGE和Western blotting分析表达的融合蛋白分子量约为19 kD，以不溶性的包涵体形式存在。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐