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Under the same conditions pET28a-△SIL-2 expression was higher. RNA structure software analysis showed that mRNA5'end sequences of pET28a-IL-2 formed a complex and relatively stable secondary structure, which testified that the existence of the signal peptide sequence had influence on the ChIL-2 gene in prokaryon expression.

RNAstructure软件分析表明,pET28a-IL-2的mRNA5'端序列形成了复杂且较为稳定的二级结构,不利于翻译的起始,证明信号肽序列的存在对ChIL-2基因在原核细胞中的表达有一定的影响。

According to the sequence of rabbit defensin NP-1 and porcine defensin pBD-1 gene published in GenBank,We selected respectively prokaryon or eukaryotic expression vector to construct recombinant expression plasmid hoping for getting expression protein in E.coli or COS-7 cells, All these laid down a foundation in research of these two antibacterial peptides.

本文主要根据GenBank已公布的兔防御素NP-1基因和猪防御素pBD-1基因序列,分别选用原核或真核高效表达载体构建重组表达质粒,以期在大肠杆菌或COS-7细胞中得到高效表达,为进一步研究这两个抗菌肽奠定基础。

The above results may offer a clue to produce biodiesel with procaryote.

上述结果可能为利用原核生物做生物柴油原料提供线索。

By a two-promoter vector, we successfully coexpressed DsbA-DsbC fusion protein with hNGF in procaryote cytoplasm and obtained the soluble hNGF protein, but the DsbA or DsbC alone do not show this function.

通过本课题的研究,我们根据DsbA和DsbA~蛋白的性质特点获得了一种原核通用融合表达载体,该载体在表达真核来源的活性蛋白或细胞因子具有促可溶和保持目的蛋白天然活性的功能。

Function of procayon and plasmid: to carry and pass on genetic information.

原核和质粒的功能:携带和传递遗传信息。

Characterization,antigenic structure analysis of Chinese wheat mosaic virus,a new specie of genus Furovirus and expression of its RNA2 genes in E.coli

中文题名 Furovirus属新成员:中国小麦花叶病毒的鉴定,抗原结构分析及RNA2相关基因的原核表达

To optimize the prokaryotic expression of MCP gene of Red-spotted Grouper Nervous Necrosis Virus.

[目的]优化赤点石斑鱼神经坏死病毒的主衣壳蛋白基因的原核表达条件。

The organization of the ribosomal RNA genes of N.antheraeae is LSU-ITS1-SSU-ITS2-5S,a pattern similar to those of N.bombycis.

柞蚕微孢子虫的核糖体基因排列顺序为LSU—ITS—SSU—ITS—5S,和家蚕微孢子虫的排列顺序相同,是一种即不同于真核生物,也不同于原核生物的排列顺序。

AIM摘要: To construct the recombinant plasmids expressing fulllength HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E.coli.

目的摘要:构建丙型肝炎病毒全长及3种不同缺失突变的核心蛋白基因的原核表达载体,并在大肠杆菌中表达。

More and more studies indicated they will play a large role in modern taxonomy of prokaryotes.

这些分类方法必将在原核生物现代分类中扮演举足轻重的角色。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。