原核

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Expression vectors pET-TPO and pBV-TPO were constructed to express full-length TPO cDNA in E.coli . Influence of SD-AUG and promotors on the expression level was studied.

在克隆并分析了TPO cDNA的基础上，将全长hTPO cDNA克隆到原核表达载体pET-15b和pBV220中得到了表达，并且以pBV220为载体探讨了SD序列与AUG之间的距离对cDNA表达效率的影响；为了进一步提高表达效率，将hTPO cDNA 5？

B The HCBP12 fusion protein was highly expressed in the prokaryote expressing system.

HCBP12融合蛋白在原核表达系统成功表达。

This phenomenon is quite common in prokaryote, bacteriophages and viruses as an economical and effective way to present the genome information.

双重编码现象在原核生物、噬菌体和病毒中作为一种可有效利用基因组的方式而普遍存在。

In chapterⅡ, knowledge of biology related to this study is explained, and the replication mechanism of prokaryote genome is illuminated.

论文第二章重点介绍论文中涉及的生物学背景知识，阐明了原核生物染色体复制机制。

OKP-B-13 gene can be expressed in prokaryote cell, which may provide foundation for further analysis of this novel β-lactamase.

结论β-内酰胺酶OKP-B-13在原核表达细胞中实验了基因重组表达，为进一步分析酶的特性提供条件。

The purpose of this test is to reveal the mutagenic potential of a substance in a prokaryote organism and whether the reactive metabolite is a product of metabolic activation by mammalian enzymes.

这次试验的目的是揭示在原核微生物的诱变物质的潜力和活性代谢物是否是一种代谢酶的激活产品的哺乳动物。

SHV-59 gene can be expressed in prokaryote cell, which laid a foundation for further analysis of this novel β-lactamase.

结论β-内酰胺酶SHV-59在原核表达细胞中实验了基因重组表达，为进一步分析酶的特性提供条件。

The prokaryote E. coli is most elaborated as a heterologous host, because most of the genes were already expressed in the strain for functional analysis.

该原核生物大肠杆菌是最阐述了作为异种主机，因为大部分的基因，已经体现在应变功能分析。

The construction of expressed plasmid in prokaryote not only obtained abundant protein but done research further for its separation, purification and biological activity.

构建斑马鱼GnRH原核表达质粒不仅获得大量的GnRH蛋白，还可对该蛋白做进一步分离纯化以及生物活性方面的研究。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐