原核

This animation was built as a "hybrid" of prokaryote and eukaryote replication proteins and dynamics.

在这段影片中，我们假想了一个原核细胞的复制蛋白和真核细胞的动力学&混血儿&。

Objective: To clone and express human MAGE-C2 gene and to investigate the expression pattern in transfected eukaryote cells.

目的：克隆人黑色素瘤相关抗原MAGE—C2的cDNA，构建真核、原核表达载体并转入相应细胞获得表达。

Which of the following methods of gene regulation do eukaryotes and prokaryotes appear to have in common?

在下列方式中，真核细胞和原核细胞的基因调节方法有何共通之处？

This has led to the theory that eukaryotes arose as a result of symbiotic associations between prokaryotes.

这就形成了真核生物中某些细胞器来自于共生的原核生物的理论。

It was demonstrated that the Vp54 gene promoter has activity in both prokaryotes and eukaryotes.

这说明Vp54基因的启动子在原核和真核生物中均具有启动活性。

My view is that there is no such dichotomy in biology between macroorganisms and microorganisms or prokaryotes and eukaryotes.

我不认为生物学应存在宏生物与微生物或者原核生物与真核生物的鸿沟性分离。

Prokaryotes are relatively simple, eukaryotes are more complex in structure and function

原核细胞的结构和功能都及其简单，而真核细胞就复杂的多

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Component of prokaryotic RNA polymerase holoenzyme.

原核生物RNA聚合酶全酶的组成成分。

Comparison of the coding regions of BH1-BH4 genes showed that BH1 had a lower sequence identity to other previously published B-hordeins than the other three B-hordeins obtained in this study. BH1 was then cloned in a bacterial expression vector based on bacteriophage T7 RNA polymerase. The resulting plasmid produced a 28.15 kDa protein in Escherichia coli. The potential value of B-hordein genes in grain quality improvement of hull-less barley has been discussed.

并且，在4个基因BH1～BH4中，BH1与先前报道的B组醇溶蛋白基因有较低的序列相似性，因此我们对BH1基因进行了原核表达，含该基因的表达载体在大肠杆菌中表达出相对分子质量为28.15 kDa并以包涵体形式存在的蛋白，进一步对其在青稞谷粒品质改良中的潜在价值进行了探讨。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐