原核

Bacteria A kingdom containing all the prokaryotic organisms, formerly called Monera or Prokaryotae.

细菌：包括所有原核生物体的一界生物，以前叫做原核生物界。

We concluded that the Spidroin2 cDNA of Nephila clavipes can be expressed in prokaryotic expression system, and addition of exogenous alanine plays important role in the promotion of expression of the naive gene of spider silk protein.

本研究证实，蜘蛛牵引丝蛋白Spidroin2 cDNA可在原核细胞内正确表达，外源丙氨酸的加入对于提高天然蜘蛛丝蛋白基因在原核系统的表达作用明显。

The main objective of the dissertation was to improve and modify the experimental procedures of mouse nuclear transfer and pronuclear transplantation, respectively, and try to establish new possible methods for mouse cloning and pronuclear transplantation.

本论文的主要目的是改进和修改小鼠核移植和原核置换的实验程序，试图建立新的小鼠核移植和原核置换的可行方法。

The extra pronuclear of triploid zygotes after IVF is from sperm and the zygotes have no implantation value.

IVF后3PN卵子中多余的原核多为精子来源，没有移植价值；ICSI后3PN中多余的原核非精子来源，ICSI后3PN中二倍体率与IVF相比显著增高，在患者ICSI后无2PN卵子时可考虑行植入前遗传学诊断。

In this study, a new horizontal microinjection system has been established to solve these problems. Because the average time per pronuclear embryo by microinjection has been significantly shortened with the horizontal system, the cleavage rate and 16-cell rate of those microinjected embryos increased markedly.

为了解决这些问题，本研究有针对性地设计了新型的水平微注射系统，利用此系统，可明显缩短常规注射原核胚的平均时间，注射后原核胚的分裂率、8-16细胞胚胎发育率也明显提高。

To pronuclear formation rate after oocyte in vitro maturation 18 hours and parthenogenticactivation, oocytes derived from SM is significantly higher than oocytes from PM,oocytes derived from IM is significantly lower that of oocytes detrived from PM and SM.

对于孤雌刺激反应而言，无论是何种类型的卵母细胞成熟18小时后，自发成熟卵母细胞激活后形成原核的比率高于被动成熟的卵母细胞，诱导成熟的卵母细胞的原核形成率显著低于自发和／或被动成熟的卵母细胞。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

Compared genes up- and down-stream IL-21 between Tetraodon nigroviridi, Fugu rubripes and human, a high degree of conservation of synteny was found.

将TnIL-21 CDS序列克隆到原核表达载体pET32b中，成功地构建重组载体TnIL21-pET32b，并建立其原核表达体系，成功诱导目的蛋白的大量表达。

The recombinant protein were purled from prokaryotic bacteria hadactivity of binding to IBV in ELISA and Western blotting test, which could be blocked byantiserum against gAPN.

将原核表达的gAPN蛋白进行的ELISA及Westem blotting试验证实原核表达的gAPN蛋白在体外可以与IBV结合，而且这种结合可以被针对gAPN的抗体阻断。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失