原核

Objective To perform prokaryotic expression, purification and identification of Xenopus laevis and mouse vascular endothelial growth factors.

目的 构建非洲爪蟾和小鼠血管内皮细胞生长因子重组表达载体，进行蛋白质原核表达，并对表达产物进行分离、纯化和鉴定。

Antiserums against the coat proteins were prepared by prokaryotic expression and 3 by virus purification.

原核表达制备了27种线状植物病毒的外壳蛋白抗血清，提纯病毒制备了3种线状植物病毒的外壳蛋白抗血清。

Antiserums against the non-structural proteins of those viruses were also prepared by prokaryotic expression.

原核表达制备了24个功能蛋白的抗血清。

Using Perl as programming language and integrating databases technology, microsatellites, longer than 12 nucleotides in length, in 29 eukaryotic and 1180 prokaryotic genomes were computed and censused in great scale.

本研究采用Perl为编程语言结合数据库技术等方法，对29个真核生物基因组和1180个原核生物基因组的DNA序列上长度超过12的微卫星进行了大规模的计算和统计分析。

It is on the large surface area provided by the inner cristae of mitochondria that ATP-generating

线粒体自我复制，并且可能是自由生活的原核生物在进化中形成的后代。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

Lower termites ; Mutualism ; Flagellates ; Prokaryotes ; Diversity ; Function

低等白蚁；共生；鞭毛虫；原核生物；多样性；功能

The gut of lower termites harbor a complex microbial community,including eukaryotic flagellates and prokaryotic bacteria and archaea,which play important roles in the wood-cellulose digestion of these termites.

低等白蚁肠道里存在着复杂的微生物区系，包括真核微生物鞭毛虫和原核生物，细菌及古细菌。

Swine hemagglutinating encephalomyelitis virus ; Hemagglutinin esterase protein ; Cloning ; Prokaryotic expression

猪血凝性脑脊髓炎病毒；血凝素酯酶蛋白；克隆；原核表达

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失