原核

To clone the site A of S gene of transmissible gastroenteritis virus and to construct the prokaryotic expression vectors of site A.

对猪传染性胃肠炎病毒S基因A抗原位点进行克隆和原核表达载体的构建。

Objective To obtain the recombinant protein of an antigen gene Ts88 of Trichinella spiralis and identify the characteristics of the recombinant protein.

目的 原核表达旋毛虫抗原基因Ts88，并对重组蛋白的抗原性进行鉴定。

Objective To express the antigen gene Ts21 of Trichinella spiralis , purify the recombinant protein and test its antigenicity.

摘要目的原核表达旋毛虫相对分子质量21 000抗原基因（Ts21）并对重组蛋白进行纯化和抗原性鉴定。

Accordingly, eukaryotic microorganism cells would die. In the experiment, Staphylococcus aureus was employed as the representative of prokaryotic microorganisms, and Candida albicans and Trichophyton mentagrophytes as the representative of eukaryotic microorganisms, respectively.

在实验中，作为原核微生物的代表使用金黄色葡萄球菌，作为真核微生物的代表使用了白色念珠菌和须癣毛癣菌，并对各种类进行了检测对比。

The expression of the unfused protein in the strain E.

将PoIFN-α1a插入到原核表达载体pGRW，转化大肠杆菌DH5α，得到的重组菌株经诱导有以包涵体形式表达的目的蛋白条带出现，表达量约10％。

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

There was still some hope for anyone who wished to uphold the simple, prokaryotic picture of unsplit genes.

对于那些希望坚持基因都象原核生物那样不可分裂的简单理论的人来说，仍然存在着一线希望。

There was still some hope for anyone who wished to uphold the simple, procaryoutic picture of unsplit genes.

对于那些愿意抱着基因都象原核生物那样不可分裂的简单理论不放的人来说，仍然存在着一线希望。

In order to test the antibodies to E6 and E7 induced by the recombinant vaccina virus, the fusion proteins of GST and E6 or E7 were expressed in E. coli using pGEX-2T vector. The expressed proteins were confirmed by Western-blot and purified by glutathione sepharose CL-4B affinity chromatography. The purified proteins were used to detect the anti-E6 and-E7 by ELISA.

为便于检测重组痘苗病毒所诱发的E6和E7抗体，本文利用原核表达载体pGEX-2T，在大肠杆菌中分别高效表达了GST与E6或E7的融合蛋白，并利用纯化的融合蛋白成功地建立了E6和E7抗体的ELISA检测方法。

Certain eukaryotic cells can swim freely in liquid environments, propelled by whiplike cilia or flagella.

某些原核细胞能够在液体环境中自由游动，由鞭子似的纤毛和鞭毛推进。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐