原核

The healthy survivals were also transferred into the oviducts of pseudopregnant female mice. PCR was used to analyze the integration of the transgene in the genomes of mice.

选用内交系C57BL／6J种小鼠作为受精卵的供体鼠，昆明种小鼠作为假孕母鼠，将长2583bp、携带有CMV启动子和人CKLF-1基因的片段显微注射到受精卵的原核内，然后将注射后成活且健康的受精卵移植到假孕母鼠的输卵管内使其发育。

The vector was linearized and injected into 817 fertilized eggs of mice in which coagulation factor IX gene has been knocked out. The manipulated embryos were transferred into the oviducts of 45 pseudopregnant females, from which 63 offsprings were obtained.

将其线性化后，用显微注射法注射入817只凝血因子Ⅸ基因剔除小鼠受精卵雄原核，再将它们分别回输45只假孕受体母鼠的输卵管中，共产仔69只，存活63只。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

Objective To construct an expression vector for fusion gene of heat-liable enterotoxin B subunit and major outer membrane protein of Chlamydia psittaci and express the fusion gene in prokaryotic cells.

目的构建含大肠杆菌热不稳定肠毒素B亚单位和鹦鹉热衣原体主要外膜蛋白融合基因的表达载体，并在原核细胞中表达融合蛋白。

Chlamydia psittaci ; Escherichia coli heat-liable enterotoxin B subunit ; Major outer membrane protein ; Prokaryotic expression

鹦鹉热衣原体；大肠杆菌热不稳定肠毒素B亚单位；主要外膜蛋白；原核表达

In recent years, both HeV and NiV have continued to emerge and reemerge, The epidemic investigation suggested that the virus could be widely present in the Flying foxes, especially Pteropus lylei in Tailand and Cambodia, which are close to China, moreover, the similar enviorment and Flying foxes species、distribution in the south-east area poese a great threat to pig industry and public health of China, based on the above issues, there is a pressing need for diagnostic technologies on the two virus.

为有效防治亨得拉病毒和尼帕病毒在我国的发生和流行，本研究首先利用原核表达系统成功地表达HeV N、NiV N基因以及NiV G一段主要抗原区基因GC，并证实了两种N蛋白的主要抗原区位于C末端约200aa区域。

The successful cloning and expression of yak IL2 gene is putatively useful for the development of novel immunopotentiator to raise the immunity of yak against various infectious pathogens and the immuno-protective efficacy of vaccinations.

本实验成功克隆了犛牛IL2基因，其原核表达产物具有显著的免疫活性，这为研制新型免疫增强剂来提高犛牛对各种疾病的抵抗力，增强犛牛疫苗的免疫保护率奠定了基础。

XC7201 is an interesting protein comprising 176 amino acids. Many sequences in the prokaryote and eukaryote kingdom with high similarity score were detected by BLAST search. It was predicted to a 3-hydroxyanthranilate 3,4-dioxygenase (3HAO), which is an extradiol dioxygenase that catalyzes the final conversion step of tryptophan to quinolinic acid in the kynurenine pathway.

XC7201由176个胺基酸所组成，比对已知物种资料库后发现在原核真核菌中都有类似的序列，其功能被预测为3-hydroxyanthranilate 3，4-dioxygenase (3HAO)。3HAO是一种双氧化酵素，在生物体进行把Tryptophan转变成Quinolinic acid的生合成途径中最后一个反应的催化。

Objective: To construct the prokaryotic expression vector of human cancer-testis gene NY-ESO-1 by the method of LP Recco PCR Cloning.

目的：利用PCR产物靶向克隆法构建人癌－睾丸抗原NY-ESO-1的原核表达载体。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失