原核

Even though the older oocytes were easier to activate and developoed more rapidly to the pronuclear stage than younger ones after artificial stimulation, the percentage of blastocyst development was lower than that in younger oocytes.

大龄卵比小龄卵易于激活而且形成原核的速度比小龄卵快，但卵激活后发育至囊胚的比例较小龄卵低。

The Ca〓 in PbⅡ stage oocyte was mainly arround pronuclear.

在第二极体阶段原核内Ca〓分布较少。

Furthermore, with only a half -dose of DNA in the current system, by the horizontal system under×100 magnification both the survival rate of pronuclear embryos following microinjection and the integration frequency of exogenous DNA injected into embryos also increased.

此外，利用该系统还可在低倍镜下×100）将DNA常规注射量减半注射，提高了原核胚注射后的成活率，其注射胚的外源基因整合率亦明显提高。

The fertilized egg at the pronuclear stage (prior to the first cell division) is then placed into the fallopian tube hoping that the tubal environment, just as in GIFT, will aid in embryo development, growth and pregnancy.

将原核期的受精卵移植回输卵管环境，此时就同GIFT一样了，这将有助于胚胎的发育。生长和妊娠。

Intracytoplasmic sperm injection is a very important assistant reproductive technology for treatment of man infertility and fundamental research , Success in ICSI in human and other animals has recently been achieved., The present pilot studies were conducted to establish a preliminary procedure for ICSI in mice, explore the experimental conditions of ICSI in mice, investigate factors influencing the test results, and accumulate technologic basis for the next studies on pronuclear exchange in mice .

本部分研究论文初步建立了小鼠ICSI技术的基本操作程序，摸索了小鼠ICSI研究的操作条件，总结了一些影响显微注射效果的重要因素，并为下一部分小鼠原核置换的研究奠定了雄厚的技术基础。

HEPO ; Milk ; goat ; Mammary gland ; expression ; Pronuclear microinjection

hEPO ；奶山羊；乳腺表达；原核注射

Icrofilaments were mainly distributed in the cortex area which chromosomes located at mataphase of second meiosisand concentrated around pronuclei after pronuclear formation.

II 期卵母细胞微丝主要集中在染色体所在的皮层区域，原核形成后微丝绕核重新聚集。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析，结果显示：(1)小鼠卵母细胞的乙醇激活结果表明，随着乙醇浓度的升高和作用时间的延长，卵母细胞激活率和发育率都显著提高，但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加，7%乙醇作用卵母细胞7min 为最佳激活条件。

Protozoa is a special and independent kingdom of microorganism in theprocessing of the evolution from prokaryote to eukaryotic cell.

原生动物是区别于原核生物和高等真核生物的一个特定类群。

No embryos developed to term after transfer of the pronuclei stage embryos to oviducts of pseudopregnant mice.

将体外受精得到的重组卵来源的双原核胚胎进行输卵管移植，没有胚胎发育到期。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失