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So far, the mammalian pronuclear transplantation is mainly studied on mice.

迄今为止,哺乳动物的原核置换研究主要在小鼠上进行。

A total of 38 males and 24 femals irradiated at the pronuclear stage survived and were available for testing.

原核期受照中共有38只雄性和24只雌性存活下来,并可供试验。

So, firstly we studied pronuclear embryo of rabbit by sequence culture that is a more developed in vitro culture system.

因此,我们首先对兔原核期胚胎采用一种较先进的体外培养系统——序贯培养进行了研究。

The technique of pronuclear microinjcetion is the most reliable and popular method for transgenic livestock production to date.

到目前为止,原核注射是最可靠,也是使用最广泛的动物转基因方法。

The relationship between cytoplasm and nucleus is the basic problem of biology, and nuclear transfer and pronuclear transplantation are the important methods to research their relations.

细胞核与细胞质的关系是生物学的基本问题,核移植和原核置换是研究核质关系的重要方法。

We compared the effects of ICSI with and without a combination of DTT and EA on male pronuclear formation and embryonic development of porcine oocytes.

比较DTT与电激活的联合处理或单一处理对ICSI后猪卵母细胞雄原核形成与胚发育的影响。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中,经Southern blot法检测有4头羊含有hEPO基因,其中3头为母羊,1头公羊于出生后20d死亡;3头转基因母羊hEPO基因的拷贝数分别为1、10、2;Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa;MTT法检测结果表明,在泌乳10d的3只转基因羊乳汁中,每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

Sperm morphological change after ICSI and factors affecting the pronuclear formation and embryo development of buffalo oocytes after ICSI were investigated in this study.

本研究主要探讨水牛胞质内受精后精子的形态变化、原核形成类型及相关影响因素。

The cleavage and pronuclear formation rate of oocytes treated with 5μmol/L Ion were significantly higher than that of oocytes with EH treatment.

结果发现,5μmol/L Ion激活处理水牛卵母细胞分裂率(63.0%vs 54.2%,P<0.05)及原核形成率显著高于EH处理的卵母细胞。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后,分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h,研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。