原核

Secondly, although protein sequence hydropathy prediction result showed ZmDWF1 is an integrated membrane protein, we still successfully expressed the full-length protein fused into the plasmid pET30a , then purified the 70 KD fusion proteins by Ni-NTA agarose affinity chromatography column.

其次，尽管蛋白氨基酸序列的疏水性分析表明，ZmDWF1是一种整合膜蛋白，我们依然将ZmDWF1全长序列克隆到原核表达载体pET30a中，并成功的在大肠杆菌BL21（DE3）中表达了这种预测的膜蛋白全长。

IL-18-PE38 recombinant immunotoxin expression vector will provide the basis for study on the targeted cytotoxic activity to Th1 cells and may have some potential value in the treatment of Th1 cell-mediated autoimmune diseases.

结论获得了IL-18-PE38融合基因在原核系统的表达，为进一步研究其对Th1细胞的靶向细胞毒性及临床应用奠定了基础。

Methods: Clone the genes of ScFv (OC183B2) and PE38 into PET30a and then express the immunotoxin after induced with IPTG.

采用基因工程技术，经过3次亚克隆，将编码单链抗体的基因及编码毒素的基因亚克隆至原核表达载体pET30a上，并在IPTG的诱导下进行表达。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

Form、structure、characterization and function of prokaryotic、eukaryotic and molecular organisms;②nutrition and cultivation techniques of microorganisms;③physiological and metabolizable production of microorganisms;③theory of genetic variation and microbic breeding technique of microorganisms;⑤ecology and environmental protection of microorganisms;⑥Infection、 Immunity and biologics of microorganisms;⑦classification and identification of microorganisms and the international Code of Nomenclature of microorganisms.and microbial high-techs developing on these as the fundament of life sciences.

主要讲授：①原核微生物、真核微生物及分子生物的形态、结构、特点和功能；②微生物的营养和培养技术；③微生物生理及代谢产物；④微生物遗传变异理论和育种技术；⑤微生物生态和环境保护；⑥微生物传染、免疫和生物制品；⑦微生物分类鉴定和国际命名法则，以及在此基础理论上发展起来的微生物高新技术，是当代生命科学研究的基础。

Four iertransform infrared microspectroscopy was used to investigate the effects of C-terminal acidic protein on the secondary structure of wheat α-thionin in the absence of signal pepti deduring the prokaryotic expression process.

采用傅里叶变换显微红外手段研究了在信号肽缺失的情况下，C-末端酸性蛋白的存在对小麦α-硫素原核表达过程中蛋白质二级结构的影响。

Sequence analysis showed that the size of 16S rRNA gene was in 1235 bp, and lacking several regions which regarded as eukaryotic sequence, was more similar with prokaryotes than eukaryotes in sequence structural feature; it could be implied that the microsporidia had split from ancient eukaryotes very early in time on the phylogenetic tree.

序列分析表明，16SrRNA基因大小为1235bp，缺乏多个被认为是真核生物序列的区域，序列结构特征更多地类似于原核生物的，在进化上，推测微孢子虫在很早以前与原始真核生物产生分歧；分子进化分析发现，同属的微孢子虫可以处于系统进化树不同的分枝。

In order to better understand their phylogenetic relationship and identify the uncertain species, the sequences of the small subunit ribosomal RNA (ssurRNA, 16 S rRNA) genes of nine microsporidia infectious to the silkworm, Bombyx mori, were determined.

微孢子虫是真核生物，但其核糖体及核糖体RNA为原核生物型。为探讨9种家蚕病原性微孢子虫的种属地位及亲缘关系，对已广泛用於生物进化分类的核糖体小亚单位RNA基因进行了研究。

Plantlike organisms include cyanobacteria in the kingdom Monera.

类似植物体包括在原核生物界的氰细菌。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失