原核

The present study provides experimental data to understand ADH enzymic property, and to explore the relationship between polymorphism of ADH activity and disease.

为进一步了解ADH的酶学性质，探讨ADH与疾病的关系，本文构建了人ADH原核表达载体，进行了体外表达，并测定了部分酶学参数。

It is a run-of-the-mill prokaryote, an eubacterium, a common inhabitant of the human intestine.

它就是一种普通(run-of-the-mill)的原核生物，一种生活在人类肠道里的真细菌。

This results, combining pathological, evolutionally biological and genetic evidences, suggested it was possible that foreign transposable elements exist widely both in prokaryotes and in eukaryotes.

并且结合许多病理学、进化生物学及其遗传学的证据对异源转座子在原核和真核生物中广泛存在的可能性进行了讨论。

The prokaryotic expression system of flagellar biosynthesis genes flhA, flhB2 and fliR of Leptospira interrogans was successfully constructed.

已成功构建了问号钩端螺旋体鞭毛相关基因且flhA、flhB2和fliR原核表达系统。

Objective To clone the flagellar biosynthesis genes fihA, flhB2 and fliR of Leptospira interrogans and construct their prokaryotic expression system Methods Extract genomic DNA from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain 56601 by phenol-chloroform method and amplify fihA, flhB2 and fliR gene fragments by high fidelity PCR.

目的 克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR，并构建其原核表达载体。

Generative nuclei The two gametic nuclei formed by the division of the generative cell in the pollen tube of angiosperms.

雄原核：在被子植物花粉管中的生殖细胞分裂成两个配子的核。

The gene of human proinsulin mutant (A21Asn→Gly,B17Leu→Ile,B31Arg,B32Arg,GIRR ) was synthesized and recombinated to the fusion protein expression vector pTXB1 and expressed in E.coli BL21(DE3) with high protein level.

构建人胰岛素原突变体GIRR（A21Asn→Gly，B17Leu→Ile，B31Arg，B32Arg）基因的原核融合表达载体并进行表达和纯化，进一步对其性质进行研究。

Result: the regions with strong to very stong labeling by the GDNF probes were as follows: the caudate-putamen, the habenular nucleuse, and some areas of the brainstem and spinal cord, the trigeminal ganglion, the dorsal root nerve ganglion.

本实验利用原位杂交技术结合RT-PCR技术观察了GDNF从E16～成年大鼠神经系统内的分布；为了更进一步研究GDNF在神经发育中的作用与作用机制，本实验还利用基因克隆技术克隆了人GDNF基因并进行了原核表达GDNF蛋白的研究。

The 15 ku ES antigen gene was amplified by RT-PCR from the total RNA extracted from the Haemonchus contortus.

contortus15ES抗原基因进行原核表达，本研究利用RT-PCR技术从黑龙江省羊源H。

Then the fusion protein was primary purified by affinity chromatography. The GST tail of the fusion protein was cleaved by Thrombin. Nucleotide sequence analysis showed that the gdh gene of S.suis possesses the highly conserved motifs typical of family I hexameric GDHs.

猪链球菌2型谷氨酸脱氢酶编码基因克隆、序列分析及原核表达根据已发表的猪链球菌2型的gdh基因设计引物，扩增海安病人分离株Habb中的目的基因，并进行序列测定和分析；构建重组表达载体pGEX4T-2-gdh，在大肠杆菌中表达，并纯化重组蛋白。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失