原核

When the porcine-porcine and porcine-mouse nuclear transfer embryos were activated,inthe former only one pronucleus was observed in most cases,but in the later two pronuclei werefound mostly.

猪-猪同种核移植胚胎和猪-小鼠异种移核胚胎激活后，异种重组胚多形成二原核(2PN)，而同种重组胚则主要形成一原核。

ABSTRACT］ObjectiveTo construct the prokaryotic expression vector of tegument protein pp85 of human herpesvirus 7 and to express it.

摘要］ 目的构建人疱疹病毒7型(HHV7)开放读码框（ORF U14的原核表达载体，并且进行原核表达。

our successful cloning and expression of human tum 5 gene and purification of rhtum 5 protein lay a basis for further study on the application of this protein to anti angiogenesis in vitro and in vivo.

成功的构建了人tum 5基因的原核表达载体，并纯化了该基因的原核表达产物。

The cloned Buchnera groEL genes of Schizaphis graminum and Rhopalosiphum maize were ligated into pBV221,pET30a and pTrcHisA expression vector for construction of pBVRM,pBVSG, pETRM,pETSGPR,pTrcRM and pTrcSG. They were transformated into E. coli BL21(DE3),DH5a and JM109 for studying their expression.

2将克隆的玉米蚜和麦二叉蚜Buchnera groEL基因连接到原核表达载体pBV221、pET30a和pTrcHisA上，构建了含2个目的基因的6个表达原核表达载体：pBVRM、pBVSG，pETRM、pETSG和pTrcRM、pTrcSG。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

Objective To construct prokaryotic recombinant plasmids of transcriptional co-activator gene of Clonorchis sinen sis, express and purify the recombinant protein and analyze its biological function.

摘要目的构建华支睾吸虫成虫转录辅激活因子(transcriptionalcoactivator ，TC)基因原核重组质粒，进行原核表达、鉴定及生物功能分析。

PDGF-BB is the most effective subtype in the PDGF family on the metabolism of osseous tissue or the treatment of wound healing, and its clinical application was approved firstly among the growth factors although it was used to treat the decubital ulcer of diabetes.

本研究通过分子克隆方法获取人血小板衍生生长因子B链成熟肽基因，构建原核表达载体；利用大肠杆菌体系原核表达人血小板衍生生长因子B链前体蛋白，并纯化、复性进而获得具有功能活性的PDGF-BB；并对获得的重组rhPDGF-BB蛋白进行体外功能活性鉴定，同时，初步探讨PDGF-BB在毕赤酵母中的表达，为寻找更优化的表达纯化方法提供实验学依据。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

A member of the kingdom Monera, comprising the prokaryotes and including all bacteria.

原核生物无核原生物王国的一员，由原核生物组成，包括所有细菌

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失