原核

In prokaryotes the nuclear material is simply a circular strand of DNA, not complexed with histone proteins, and cell division is amitotic.

在原核生物中，核物质仅是一个简单的环状 DNA ，而不与组蛋白结合，并且细胞为无丝分裂。

In comparison with the reported corresponding sequences, the putative amino acid sequence shares high homology with assimilatory nitrate reductase of other microalgae, with identity 78% to Dunaliella tertiolecta, 60% to Volvox carteri, 58% to Chlamydomonas reinhardtii, and 50% to Chlorella vulgaris, respectively.

2.3 杜氏盐藻NR基因原核表达载体在大肠杆菌中的表达和功能鉴定(1)pMAL-NR-2组大肠杆菌SDS-PAGE结果显示：在140kDa左右出现一条特异性条带，分子量与MBP-NR融合蛋白相符（BMP+NR=42.5kDa+99.5kDa=142kDa），在IPTG（0.15mmol/L）37℃诱导时，该蛋白量占全菌蛋白的21.06％；对照组无此特异带。

In prokaryotes, a means of transferring genetic information from one bacterium to another across a bridgelike structure.

接合作用：原核生物中的一种传递遗传信息的方法。它通过一个桥状的结构从一个细菌到另一细菌。

We gained the Buchnera groEL gene of Schizaphis graminum, and Rhopalosiphummaize biotype through this study , and have done a primary study of expression. Based on this study, we can further probe the role during aphid transmission of Buchnera GroEL and utilize them through the ways of genetic engineering.

蚜虫内共生菌Buchnera groEL基因的获得、初步的原核表达研究以及GroEL蛋白抗血清的制备，为进一步研究GroEL蛋白在蚜虫传毒中的作用方式和位点以及在基因工程途径的利用打下了基础。

Our study designed two pairs of specific primers and cloned two molecular weight of 63ku from Schizaphis graminum, and Rhopalosiphum maize utilizing PCR technique and have done the prokaryotic expression, the main results are as follows:(1)Amplified the Buchnera groEL of Schizaphis graminum, and Rhopalosiphum maize Yangling Biotype at first time all over the world ,and sequenced the full lenghth gene.

本研究设计了特异性引物，利用PCR技术从玉米蚜和麦二叉蚜中克隆出蚜虫Buchnera groEL基因并进行原核表达研究，主要结果如下：(1)从杨凌生物型玉米蚜和麦二叉蚜中在国内外首先扩增出Buchnera groEL基因，测定了全长基因序列，并对相关的同源基因核苷酸进行了比较。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

The study on the expression of TGF β1 gene in Escherichia coli, not only can provide great amounts of TGF β1 for its clinical and basic research, and also will bring some new ideas and means for the research of other dimeric growth factors.

开展TGFβ1基因在大肠杆菌中表达的研究，不仅有可能获得大量具有生物学活性的TGFβ1产品，从而为TGFβl深入的基础和临床研究提供可能；而且可能为其他双体生长因子的原核表达提供新的思路和方法。

Dissimilatory nitrate reduction in fungi includes denitrification and ammonia fermentation, which occurs under the conditions of limited aeration.

真菌异化硝酸盐还原途径的发现打破了反硝化仅存在于原核细胞这一传统观念。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失