原核

Oocytes injected with frozen-thawed sperm were activated by various methods, and the effect on the pronucleus formation and blastocyst formation rate were examined.

方法］冷冻精子解冻洗涤后直接应用于ICSI，ICSI后采用不同激活方法激活卵母细胞，检测其原核形成情况。

We also successfully amplified Sry from pronucleus stage embryos, which prove that embryo can be sexed by Sry PCR method using one cell instead of the minimal detectable cell amount of 4 cells proved by others.

同时由于从原核时期扩增到了Sry，本文认为胚胎的性别鉴定取单个细胞即可。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法，将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

PCR-SSCP (single-strand conformation polymorphism) was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed:(1) The pronucleus microorganism's community was similar and also had the polymorphism in each simple of various fermentation stage;(2) Different microflora had complex ecology effects of coordination and the restriction;(3) The diversity indexes of different stage of Daqu microorganisms were around 1.69~2.01, and the composition of them was stable relatively;(4) The similarity indexes were 0.67~1.00, and much higher in the approaching stages.

采用PCR-SSCP技术对浓香型大曲发酵过程中原核微生物群落结构的变化情况进行了研究，结果表明：(1)发酵各时期曲样的群落结构相似，同时也具有多态性；(2)不同微生物群落具有协同和制约的复杂生态学效应；(3)各时期曲样微生物多样性指数均在1.69~2.01之间，群落结构相对较稳定；(4)各曲样微生物群落相似性位于0.67~1.00之间，邻近时期样品间的相似性程度较高。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

AbstractThough prokaryotic cells could hardly express recombinant human beta nerve growth factor with a proper threedimensional conformation, using of E.

基于原核表达系统极难表达具有正确三级结构的重组人β神经生长因子，E。

The relationship between the proterozoic Era and explosive evolution of the life on the Cambrian period was preliminary explored by an Ecological and Evolutionary approach in this paper.

距今38亿年前地球最早生命诞生以来，至元古代早期第一个真核细胞开始出现；从无性分裂或出芽繁殖的原核生物到有性繁殖的真核单细胞生物以及从单细胞的原生动物到真核多细胞的后生动物的进化，无疑是生命进化过程中非常重要的事件。

Objective:(1) To clone and characterize the Silent information regulator2 (SIR2) homolog from the parasitic protist Trichomonas vaginalis cDNA expression library, to express the fusion protein of TvSIR2 and TvSIR2-like/GST(isolated previously in our laboratory).

构建TvSIR2和TvSIR2-like基因原核表达重组载体并表达其融合蛋白，制备抗体，Western blotting鉴定后免疫荧光法进行细胞定位。

Plant biology is the study of plants, organisms formally classified in the kingdom Plantae, and of an equal number of plantlike organisms formally classified in the kingdoms Monera, Fungi, and Protista.

植物学是研究植物的一门学问，正式分类在植物界，还有相当数目的类似植物被分类在原核生物界、真菌界及真核原生物界。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失