原核

Aim: To express and purify the PTD-XfAP fusion protein and to verify its penetrability across the blood brain barrier.

目的：原核表达、纯化PTD-XIAP融合蛋白，鉴定其血脑屏障的通透功能。

The penetrating rate was 70%～80%, and the rate of pronucleuses formation was about 30%.

穿透率在70%～80%左右，有原核卵百分率在30%左右。

Objective To construct a prokaryotic expression vector for LY1 protein phosphatase gene, and to express the gene in E.

目的 构建蛋白磷酸酶LY1基因的原核表达载体，并在E。

It is found in all photosynthetic cells of plants and protoctists but not in photosynthetic prokaryotes.

存在于所有的植物和原生生物的光合细胞中，但在能进行光能合成的原核生物中却不存在。

Each structure is surrounded by a membrane which is similar to the inner plasma membrane of procaryotes.

每个细胞器也都由一层膜包着与原核细胞的内膜浆膜层比较类似。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

In the current study, a series of cationic α-helical peptides of 20 a.a. were designed and synthesized based on four structural parameters, including charge, polar angle, hydrophobicity, and hydrophobic moment. Several methods were performed to describe the influence of these parameters on the antimicrobial and hemolytic activity, and on the permeabilizing effect on model membranes. The potential of these parameters to increase the antibacterial activity and to improve the prokaryotic selectivity is assessed.

本实验利用自行设计合成的人工抗菌胜肽当模型，固定长度为 20 个胺基酸，改变其电荷、极性角度、疏水性及疏水性矩等四种结构特性，并利用其对各种不同病原菌之抗菌活性及对兔子红血球细胞的溶血活性来研究其结构特性的改变对其抗菌活性、选择性及微脂体膜的渗透性之影响，以期望改变结构参数能增加抗菌活性及对原核的选择性。

Objective To investigate the relationship between the first polar body morphology of oocyte and the zygote morphology in pronucleus stage, embryo quality in cleavage stage.

目的 探讨卵细胞第一极体形态与原核期受精卵形态、卵裂期胚胎质量的关系。

These results indicate that,(1) appropriate concentration of CHX can enhance the activation efficiency of oocytes;(2) CB can increase the two pronuclei formation rate of oocytes by inhibiting the extrusion of second polar body.

这些结果说明：(1)适当浓度的CHX能提高卵母细胞激活效果；(2)CB能抑制卵母细胞激活后第二极体排出，从而提高原核的形成率。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失