原核

The efficiency of transgenic animal production by the traditional method ofpronuclear microinjection is very low.

以传统的原核显微注射法生产转基因动物的效率十分低下，严重地制约了转基因动物研究的进一步发展。

The microinjection work is still going on.

目前原核注射工作仍在紧张进行中。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞，获得重组杆状病毒rBV-σC；SDS-PAGE和Western-blot分析表明，在sf-9细胞中表达了分子质量约37ku的σC蛋白，该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应，这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Each of the two genes was expressed by high-efficient expression-vector pBV220, two expression products were of Chorismate mutase/Prephenate dehydratase or DAHP synthetase activities.

用高效原核表达载体pBV220分别实现了分支酸变位酶／预苯酸脱水酶和抗反馈抑制的DAHP合成酶的高效表达，两种表达产物都具有生物学活性。

In prokaryotes the hereditary material is found in the nucleoid.

在原核生物的遗传物质存在与类核中）。

In prokaryotes athe hereditary material is found in the nucleoid.

原核细胞的遗传物质则存在于拟核上）。

A.Packaging: Prokaryotes have a nucleoid region whereas ekukaryote have a true, membrane-bound nucleus.

包被：原核细胞只有类核区；而真核细胞具有由核膜包被的细胞核。

Objective To clone human ornithine decarboxylase antizyme mutation gene and express its recombinant protein.

目的 构建人鸟氨酸脱羧酶抗酶突变基因，重组至原核表达载体中并表达出重组蛋白。

Results showed that:(1) a wide variation of overrepresentation of nucleotides exists in the MSC genes; Some genes are G+C rich, some others are A+T rich, while the rest do not show obvious nucleotide bias.

结果显示：（1）原核生物MSC 基因的核苷酸组成存在较大的变异。

Producing PHAs,and then were orientationally inserted into a prokaryotic expression vector pBV220. By analyzing the bioactibity and function of their products, the biological function of phaA, phaB and phaC was confirmed. These have been done in our laboratory. To make better use of the three genes by plant bioreactor, two groups of different plant expression vectors that can be used to produce PHAs in different plants were constructed, and the transformation of PHA in Oxalis triangnlaris has been preliminarily reseached.The following is the main results:Three plant expression vectors pBI121-A,pBI121-B and ubquiting-C, which can be used to be transformed into Oxalis triangularis were respectively constructed by using the phaA, phaB and phaC.

菌株的亚克隆基因组片段中，分离出phaA、phaB和phaC三个基因片段，定向克隆至原核表达载体pBV220，通过对其生物活性与功能分析，确证了基因phaA、phaB和phaC的生物学功能；为了通过植物生物反应器对这三个基因进一步研究利用，本实验利用这三个基因构建了两组可用于不同植物生产PHA的真核表达载体，并对紫叶酢浆草进行PHA基因遗传转化的初步研究，获得主要结果如下：利用克隆的phaA、phaB、phaC三个基因分别构建了可用于转化紫叶酢浆草表达载体pBI121-A、pBI121-B、uBquiting-C；可用于玉米转化的种子特异性表达载体p7S-A、pBI121-S-B、uBquiting-C。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失