原核

The isoform hMAM cDNA consisting of 270 bp, was different from the wild type one of 279 bp due to nine continuous base pair missing. Therefore the translational product of the isoform was seen to be lack of three continuous amino acid (-79~-81, VFM) compared with that of the wild type. The homology of the wild type and the isoform are testified to be 96% in both DNA and amino acid sequences.

乳腺癌组织和乳腺癌细胞株中发现两种hMAM cDNA亚型：hMAM和hMAM，长度分别为279和270bp，二者相差9个连续碱基，其翻译产物相差3个连续氨基酸残基(-79～-81，VFM)，二者基因和氨基酸序列匹配度均为96%。cDNA片段分别插入原核表达质粒pGEX-KG，构建的质粒命名为pGEX-KG/hMAM和pGEX-KG/hMAM。

To obtain the African horse sickness virus VP7 protein, which is used to preparing serological diagnostic reagent and constructing AHS new generation vaccine, the prokaryotic expression is used to express VP7 protein.

为获得非洲马瘟病毒VP7蛋白，以用于制备AHS血清学诊断试剂并为AHS新型疫苗的构建奠定基础，笔者采用原核表达系统表达VP7蛋白。

Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

With regard to the rapidly increasing sequence data of prokaryotic genomes, accurate gene identifications and genome annotations have become more and more important.

近年来原核生物的基因体定序速度越来越快，相对地基因的鉴定以及基因的注解也变的越来越重要。

The prokaryotic expression vector pET22b and host bacterium Roestta-gami are most suitable for CM-VEGF121,which solves the problem of low expression and insolubility.

原核表达载体pET22b和Rosetta-gami宿主菌为CM-VEGF121最合适的条件，解决了其表达量低和不可溶问题。

To investigate its function, the Yeast invertase and the expressed protein were co-cultured

的全长编码序列，并通过大肠杆菌pMAL p2X 原核表达系统得到初步纯化的

Methods Regulation region of bovine β-lactoglobulin geneand full-length genomic DNA encoding hEPO were used to construct eukaryotic expression vector,and the transgenic goats were produced by pronuclear microinjection.

方法采用牛β-乳球蛋白基因调控元件和hEPO全长编码序列基因组DNA构建真核表达载体，应用受精卵原核注射的方法制备hEPO转基因山羊。

A double-antibody sandwich ELISAemploying monoclonal antibody 5G5 was developed for detection of P27 antigen of avian leucosis virus.The limit of viral antigens detected was 5?

利用原核表达的禽白血病病毒P27蛋白作为抗原制备的单抗作为包被抗体，以制的酶标兔抗P27作为酶标抗体，在国内首次建立了检测ALV抗原的双抗体夹心ELISA方法。

The journal covers research on viruses, prokaryotes and eukaryotes - both planktonic and benthic, autotrophic and heterotrophic - in marine, limnetic and brackish habitats.

该期刊涵盖了对病毒、原核生物和真核生物的研究，包括浮游生物和底栖生物，自养生物和异养生物，海洋生物、湖沼生物及半咸水生物。

The number of His-EGFP-CRP detection peaks increased after the protein was incubated with lysate of THP-1 cells.

为解决这一技术难题，构建了带his纯化标签和EGFP荧光标记的人CRP原核表达质粒［4］。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失