原孢子

Female gametogenesis in higher plants undergoes various developmental changes, including the de novo formation of germline cells, the differentiation of the megaspore mother cell, meiosis, megaspore selection, and the development of the embryosac.

高等植物雌配子体的形成涉及孢原细胞和大孢子母细胞的确立与分化、大孢子发生、功能大孢子以及胚囊的形成和发育等多种复杂调控过程。随着当代生物技术及功能基因组学的发展，近年对雌配子体发育的研究已从细胞学描述逐渐过渡到对基因和发育调控分子机理的探索。

The result indicates that spore germination shapes prothallium at keeping wet, cortrolling temperature and covering with 75% shading rate.

结果表明：在保湿、控制温度、覆盖遮光率为75%的遮阳网的条件下孢子发芽率高，能正常形成原叶体，继续培养均能进入有性世代形成孢子体幼苗，其中在山林腐殖土和壤土按1：1的比例混合的基质上最适于孢子萌发和幼苗生长，出苗量可达3500株/m2；在杭州采集的紫萁孢子萌发后形成的原叶体、孢子体均比在湖北民族学院采集的耐高温性好。

The spore germination and gametophyte development of 21 ferns are studied. It indicates that :① the development period of prothallium and young sporophyte of the same species is different due to seeding time of spores;② the optimal temperatures of spores germination and gametophyte development of 21 ferns are about 15 -24 ℃;③ the rate of spore germination of rare ferns is much lower than the ferns which occur large populations in nature;④ the treatment with GA 3 can accelerate the germination of the spores;⑤ the prothallium changes from large to small, green to yellow when young sporophyte comes out. The prothalliums of all 21 ferns die away after the appear of the 3rd leaf of sporophytes;⑥ shapes of the 1st and 2nd leaves of the young sporophyte are different from those emerged later;⑦ spores cultured in dark can not germinate;⑧ a prothallium can develop only one young sporophyte though it has many archegoniums, and the spore propagation with leaf mould substrate is an economical method.

摘 要：以腐叶土为培养基质，对 21 种蕨类植物进行了孢子萌发和原叶体发育的研究，结果表明：①不同时期播种的同种蕨类的孢子，发育出原叶体和幼孢子体所历经的时间长短不同；②孢子萌发和配子体生长发育的适宜温度约为 15 ～ 24 ℃；③稀有蕨类的孢子萌发率低，而在野外能形成较大种群的蕨类的孢子萌发率高；④用 GA 3 处理孢子可以促进萌发；⑤当原叶体上长出幼孢子体时，原叶体由大变小，由绿变黄， 21 种蕨类的原叶体都在幼孢子体上长出第 3 片叶时消失；⑥幼孢子体上长出的第 1 、 2 片叶在形态上与以后长出的叶不同；⑦孢子萌发需要光；⑧ 1 片原叶体尽管有多个颈卵器，但仅发育出 1 株幼孢子体；⑨利用腐叶土进行蕨类孢子繁殖是一种经济实用的繁殖方法。

Based on the tissue culture, the effect of N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea, 6- benzyla minoprine（6-BA） and 1-Naphthylacetic acid on spore germination, protonema develop- ment and bud differentiation in Entodon challenged was investigated in this paper. The whole process was observed and taken pictures. The results show that TDZ, 6-BA and NAA have no significant effect on the spore germination. During the process of protonema development, 1.0 mg/L NAA can promote the primary development of protonema significantly, and 0.4 mg/L TDZ promotes branches formation in inter- mediate stage of the protonema development.

：研究了3种植物生长调节剂苯基噻二唑基脲、6-苄基腺嘌呤（6-BA）、萘乙酸对密叶绢藓孢子萌发、原丝体发育及芽体发生的影响，并对整个发育过程进行了显微观察和照相，结果表明：（1）3种植物生长调节剂对密叶绢藓孢子萌发影响不显著；（2）在原丝体发育阶段，1.0mg／LNAA对原丝体初期的发育促进效果显著，0.4mg／LTDZ对原丝体发育中期分枝的形成促进效果显著，6。

The spores of Cheilolejeunea ventricosa X.L.He were cultured on MS media (without acid and vitamin, pH=6.2, with 1% agar and 1% sucrose). The sporeling development and oil body ontogeny were observed. After a 55-day-long axenic culture, young plants were obtained successfully. Sodium hypochlorite solution was used to sterilize the capsules. Our results revealed that the sterilizing treatment with 0.1% Sodium hypochlorite solution for a period of 5 seconds was optimal. Oil bodies in primary protonemata are nearly homogeneous, but distinctly compound in the leaf cells of the primary leafy shoots.

首次研究了膨叶唇鳞苔的孢子萌发、原丝体和油体发育及配子体发生的过程，结果表明：(1)使用经过改良的MS培养基（不加氨基酸和维生素，pH值为6.2，琼脂和蔗糖浓度均为1%）能在55 d左右的时间利用孢子培养获得植物体；(2)用0.1%的次氯酸钠溶液，消毒5 s是消毒孢子的最佳方案；(3)油体在孢子萌发初期为均一型，到幼叶发生后，成为可见的聚合型，但聚合的程度比野生材料要弱。

The results show that the normal process begins with archesporial cell and undergoes stages of primary and secondary sporogenous cell,microspore mother cell,dyad,tetrad,central nucleus microspore,vacuolated microspore,mature microspore,twocell pollen and threecell mature pollen.

对平流层辐射处理SP3谷子和对照CK3谷子雄性细胞发育的研究表明，雄性细胞正常发育过程从孢原细胞开始，经初生造孢细胞、次生造孢细胞、小孢子母细胞、二分体、四分体和单核小孢子中央期、单核小孢子液泡期、单核小孢子成熟期直到二细胞花粉、三细胞成熟花粉结束。

The reproductive development process of birch was described as follows: Male inflorescences extended from apical bud in early June. In later June, stamen primodium was differentiated in the bract of male inflorescence and the differentiation of bract was generated in female inflorescence. During July, anther layers and archesporial cell were differentiated in anther, then archesporial cell developed into mother microspore cell. Pistil primodium came into being at the same time. In early part of August the meiosis of mother microspore cell started. In later August, mono-nucleus microspore was formed. After September, both male inflorescence and female inflorescence were dormant.

白桦的生殖生长发育过程如下：6月初，雄花序从顶芽中生长出来。6月中下旬，雄花序苞片上分化出雄蕊原基，雌花序分化出苞片。7月，雄花花药分化出花药壁和孢原细胞，孢原细胞演化为小孢子母细胞，雌花序苞腋处分化出雌蕊原基；8月中上旬，小孢子母细胞减数分裂，8月下旬形成单核小孢子；9月后雄花序以单核小孢子状念越冬，雌花序以雌蕊原基状念越冬。

In heterosporous plants, the microspores give rise to small male prothalli bearing male sex organs, and larger female sex prothalli bearing female sex organs.

在具异形孢子的植物中，小孢子形成具小的雄性器官的雄原叶体，而大孢子形成大的具雌性器官的雌性原叶体。

In this paper the authors report on the biological characteristics of Al-sophila spinulosa Hook.

孢子—原叶体—孢子体的生活周期长，并且在丝状体、原叶体和幼孢子体阶段，无特殊结构去抵抗和忍耐一切不利环境因子的影响。3。

If continuously culture, all can enter the sexual generation and shape sporophytic young seedling.

继续培养均能进入有性世代形成孢子体幼苗，其中在山林腐殖土和壤土按1：1的比例混合的基质上最适于孢子萌发和幼苗生长，出苗量可达3500株／m^2；在杭州采集的紫萁孢子萌发后形成的原叶体、孢子体均比在湖北民族学院采集的耐高温性好。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。