原始细胞

The results indicate that day 5 p.c.primitive ectoderm cells are able to participate in embryogenesis after be-ing introduced into day 4 p.c.blastocysts and result in the production of adult chimeras.

嵌合体与C_(57)BL/6 J小鼠交配后所得的结果表明，原始外胚层细胞在嵌合体内能形成有功能的配子。上述结果说明，原始外胚层细胞与内细胞团细胞、体外培养的胚胎干细胞一样，具有发育全能性。

BACKGROUND: The technique of isolation and culture of human scalp dermal papilla cells is basic to study hair follicle biology. At present,microdissection to isolate the dermal papillae and culture DP cells is a primitive method,which has many drawbacks,such as laboriousness,low efficiency,uneasy adhesion and poor growth.

背景：毛乳头细胞的分离培养技术是研究毛囊细胞生物学的基础，目前采用的显微解剖法分离毛乳头进行毛乳头细胞传代培养是一种原始的方法，存在着分离效率低、劳动强度大、毛乳头贴壁率低、细胞生长缓慢等缺点。

The survival time of these mice were observed. The count of white blood cells and the percentage of immature granulocyte and monocyte obtained from tail vein were determined on 1 day before transplanting WEHI-3 cells and 7, 14, 19, 24, 28 days after transplanting. Myeloblast and monoblast percentage of bone marrow were checked as soon as these mice died.

BALB/c小鼠分别经尾静脉和球后接种1×10^6 WEHI-3细胞，观测其生存天数，光镜监测BALB/c小鼠外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率，电镜观测其骨髓白血病细胞形态。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达，由原始生殖细胞产生卵原细胞，髓质退化成为卵巢腔，分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加，它们在分化结束后解体，分化前细胞内有较多的大颗粒，在分化中大颗粒减少，小颗粒增多，在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜，髓质有许多睾丸管构成，管腔内有散落排列的精原细胞。

The result showed that mouse fetus liver cells condition medium,which can maintain survivance and proliferation of mouse EG cells,was better than the medium containing no cytokine,but worse than EG cell medium with 1 000 IU/mL of LIF.

同时，试验对比了从不同胎龄胎儿分离培养原始生殖细胞的情况，鉴定了EG细胞的生物学特性；EG细胞经体外培养，可以分化为神经样细胞、上皮样细胞和简单类胚体。

Osteosarcoma ; Osteoblast ; Chondroblast ; Fibroblast ; Primitive mcsenchymal cell ; Multinucleated giant cell

骨肉瘤；骨母细胞；软骨母细胞；纤维母细胞；原始间叶细胞；多核巨细胞

We suppose that there is a presently undefined subfraction of stem cell population that exists in a number of tissues beyond embryonic development. We term them postembryonic pluripotent stem cells. They differ from embryonic stem cells in that they gradually lose some differentiation potentials during development and adopt some special phenotypes once within a certain tissue. They can give rise to different kinds of stem cells; therefore, help to maintain balance during development and metabolism. They provide cells not only for the damaged tissues they reside, but also for damaged tissues different from them by migration triggered by proinflammatory cytokines or growth factors.

我们认为在胚胎发育后的多种组织中都存在一类原始干细胞群体，它们和造血干细胞、神经干细胞等多能干细胞不同，后者只能分化为特定胚层的细胞，而这类原始干细胞可分化为不同胚层的组织细胞；另一方面，它们区别于胚胎干细胞，在妊娠期间会逐渐失去部分分化潜能，并且会出现一些特殊表型或者分子标志，我们称其为胚胎后亚全能干细胞，这类干细胞具有下列特性：可分化为不同胚层的细胞，分布于身体的所有组织和器官，具有相同的表型或标志；他们在胚胎期存在于一些或全部的组织和器官中，可分化为不同类型的多能干细胞并在生长发育和新陈代谢中维持这些多能干细胞保持平衡；可以参与机体自我修复和更新，不仅能分化为所在器官的组织特异性细胞，参与器官重塑，也可以在炎症因子或者生长因子的趋化作用下，远处转移修复受损组织。

At 4 wk, the cells in the liver bud migrated into the domain of transversum mesenchyme, where endothelial cells began to form vascular structures, and branched to form a number of hepatic cords.

结果：3wk时靠近肝芽的间充质中的血管内皮细胞多，而肝芽内未见血管内皮细胞及造血细胞：4wk时，肝索形成处可见较多正在形成的新生血管增多，肝索间开始出现少量的造血细胞：5wk时肝索间出现原始的肝血窦结构，血窦内造血细胞的数量较4wk明显增多。

Of CBMC can be purified by Mini-MACS as CD34〓 stem cells. B. The number of CD34〓 stem cells can expand to 40.24±9. 86 fold after 14 days. C. No matter in the expression of CD1a, CD80, CD86, and HLA-DR, or in the function of stimulating xenogenous lymphocyte proliferation, there was no difference between CD34+ stem cell DCs or monocyte DCs. D. The percentage of CD3〓CD56〓 cells is the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. E. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 RNA.

结果：1、Mini-MACS分选系统可自CBMC中提取0.78±0.31%的CD34〓细胞；2、体外培养14天后可获得原始CD34〓细胞量40.24±9.86倍的细胞；3、不论在CD1a、CD80、CD86及HLA-DR的表达上，或是刺激异体淋巴细胞增生的功能上，脐带血CD34〓细胞与单核细胞来源的DC都没有差别；4、CIK细胞中CD3〓CD56〓双阳性的表达率在与RNA转染DC共培养的CIK细胞组、与DC共培养的CIK细胞组及单纯CIK细胞组3组间比较无差异；5、脐带血CIK细胞增殖显著，培养14天时可扩增18.18±5.59倍，培养21天时可扩增35.02±6.30倍；5、与未转染或转染DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

First, rebuilt the concept of Cellular Consciousness of Marxism philosophy , and then putted forward the concept of Cellular Consciousness by analyze and sum-up to live cellular being activities. Secondly, expatiated on the evidence of biology and idiographic effect that the Cellular Consciousness is existence, and then demonstrated syllabifies the connection between the Cellular Consciousness and the Original Cellular Consciousness.

首先对马克思主义哲学的意识概念给予重新认识，再通过对细胞在其生命时期的诸多生命活动进行归纳和总结，从而提出细胞意识的概念；又通过详细地阐述细胞意识存在于单细胞生物及多细胞生物体内的生物学依据及其所发挥的具体作用，从而清晰地论证细胞意识与原始意识之间的相互关系，以详尽地指出细胞意识存在的巨大意义。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。