原始生殖细胞

During 6～15 week, human gonadal ridges also had hTERT positive cells.

不同培养液对人原始生殖细胞原代培养的影响。

Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differentiated.

体外分化采用无黏附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

At the fifth passage, two cell strains were made into cell suspension at l×10^9L^(-1). 0.5 mL was subcutaneously incubated at the inguina of each rat for 8 weeks. Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differeotiated.

体内实验取连续传5代的2个细胞株，分别制备浓度为l×10^9L^(-1)的细胞悬液，于每只小鼠两侧腹股沟处皮下各接种0.5 mL，培养8周体外分化采用无私附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

To investigate the proper conditions and affecting factors in culturing human primordial germ cells in vitro .

探讨人原始生殖细胞的体外分离培养条件及相关影响因素。

Comparison on the effectiveness of different cryoprotectants with chicken embryo primordial germ cells,Effect of heroin dependence on expression and function of glucagon in islet a cells of rats,Establishment of transgenic mouse line skin-specifically expressing hCTLA4-Ig,Resistance selection by meilingmycin and chlorfenapyr and activity changes of detoxicated enzymes in Tetranychus urticae.

文章摘要：鸡胚胎原始生殖细胞对不同冷冻体系适合性的研究，海洛因依赖对大鼠胰岛A细胞胰高血糖素表达和功能的影响，皮肤组织特异性表达hCTLA4-Ig转基因小鼠品系的建立，二斑叶螨对梅岭霉素和溴虫腈的抗性选育及其解毒酶活力变化，北京市褐家鼠种群遗传多态性及与汉坦病毒分布关系的研究，金乌贼同工酶分析，Nogo—A在成年大鼠脑内神经元的分布。

Primordial germ cells derived from gonadal ridges and mesenteries of 30-70-day post menopause human ernbiyos, when cultured on mouse MEF or STO fibroblast feeder layers in presence of hrLW , hrbFGF, hrSCF, and forskolin could survive and give rise to multicellular colonies, resembLing mouse ES-like colonies .

从停经30-70天流产的早期胚胎后肠系膜中或生殖嵴部位分离到的原始生殖细胞，在小鼠的原代MEF或STO成纤维细胞等饲养层细胞上培养，能在hrLIF、hrFGF、hrSCF、forskolin等的共同作用下存活并增殖。

To elucidate the mechanisms regulating TSHb expression, we isolated a 5.1-kb fragment of the red-spotted grouper 5'-flanking region by Genome walking .1.8kb of this upstream region was used to produce GFP reporter construct (pBK-TOL2) for analysis of tissue-specific expression in zebrafish embryos.

将起始密码子上游1864bp的启动子片段顺向克隆到pBK-TOL2载体中， pTSHb-TOL2重组质粒与转座酶mRNA共注射斑马鱼一细胞期受精卵，荧光显微镜观察发现，TSHb启动子驱动GFP在原始生殖细胞和垂体中表达。

During germline transposition, PB could excise precisely from original insertion sites and transpose into the mouse genome at diverse locations, pref erably transcription units.

PB因子在生殖细胞中转座时能从原始插入位点精确切离，并转座到小鼠基因组的不同位点，尤其是基因区。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失