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原外壁

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Adventitia, media and lumen cross-sectional area, wall thickness of the aorta were assessed with computed video processing.

测量收缩压和血管壁胶原含量,计算机图像分析血管外膜面积、中膜面积、管腔面积和管壁厚度。

SEM result showed that the sample had uniform phase distribution and smooth surface at dried state as well as interpenetrate network structure at hydrogel state. It was seen from the swell ing behavior curves that groups T1, T2 and T3 swelled rapidly within 10-30 minutes, and formed elastic composite hydrogel pipes. In addition, the expanding ratio of inner and outer diameter of the tube was 20%-30% and 100%-120% with swell ing, respectively. The equil ibrium water content of the hydrogel pipes increased with increase of collagen composition. Three groups of samples were immerged in PBS solution contained 2 mg/mL lysozyme at 37 for 1 month, their nozzle cracked, their wall became thin and more transparent. And also, there was small floc deposited on the tube surface.

扫描电镜观察到液氮脆断的人工泪小管内部成分分布均匀,内外壁表面平整,冷冻干燥的人工泪小管断口呈凝胶态的互穿网络结构。3 组不同成分的人工泪小管在PBS 溶液中浸泡30 min 后均快速吸水溶胀,外径扩大100%~ 120%,内径扩大20%~ 30%,并且随胶原含量增高,溶胀速度增快,平衡溶胀率增大。3 组人工泪小管在37℃含2 mg/mL 溶菌酶的PBS 液中浸泡1 个月后,表面有部分细小絮状物,管口开裂,管壁变薄,透明度增加。

According to the size and shape of oocyte, the morphology of nucleolus, the growth of yolk and the structure of follicle, oogenesis of H. d. supertexta can be divided into three stages as follows: oogonium, previtellogenic oocyte and vitellogenic oocyte. The ovary wall is composed of tunica adventitia and germinal epithelium which will produce oogonia and follicle cells. The follicle is the structure unit of ovary.

根据卵细胞的大小、形状,核仁的形态,卵黄颗粒的积累情况,滤泡的结构等,将九孔鲍卵子的发生分为卵原细胞、卵黄发生前的卵母细胞和卵黄发生期的卵母细胞3个时期;卵巢壁由外膜及内生殖上皮构成,生殖上皮分化产生卵原细胞和滤泡细胞;卵巢的结构单位是滤泡。

In early stage of tetrad,primexine produces between the plasmolemma and calloses.

四分体初期,在质膜与胼胝质之间产生原外壁,然后,在原外壁中产生原棒,进而形成了基粒棒。

Most endothelial cells fell off, elastic fibre autolyze; the number of SMC decreased, some SMC nucleus contracted completely or depressed, typical apoptosis body were seen in later stage, muscle silk autolyzed and disappeared mostly; in outer layer saw much collagenic fiber, arranged unorderly; inflammation cells were seen in the aneurysm wall(hypertrophy cell et al.).

多数瘤壁的内皮细胞溶解脱落,不连续,弹力纤维自溶;动脉瘤血管壁均可见SMC明显减少,较多SMC染色质边聚、固缩,部分SMC核全部固缩或出现不规则凹陷,SMC核胞浆或胞核部分出现脱落或裂解成碎片,晚期可见典型的凋亡小体出现,肌丝大都自溶消失:外膜可见大量的胶原纤维,排列较紊乱;瘤壁还可见炎性细胞浸润。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml,经percoll液分离后密度梯度离心,〓/ml密度接种培养,观察原代细胞的贴壁、增殖状况,细胞长满瓶底后进行传代培养;传至第三代细胞,重新消化后以500转/分钟轻度离心5分钟,〓/ml接种,改用化学限定培养基代替完全培养基培养,倒置显微镜观察细胞生长情况及形态变化,甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖,免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成,RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml,经 percoll液分离后密度梯度离心,10'砌l密度接种培养,观察原代细胞的贴壁、增殖状况,细胞长满瓶底后进行传代培养;传至第三代细胞,重新消化后以500转/分钟轻度离心 5分钟,10V加 l接种,改用化学限定培养基代替完全培养基培养,倒置显微镜观察细胞生长情况及形态变化,甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM)中的蛋白多糖,免疫细胞化学染色检测ECM中*胶原的蛋白合成,RT干CR鉴定诱导细胞*胶原mRNA的表达。

Extracellular matrix increased in the wall,resulting in collagenic and fiberous changes.

管壁细胞外基质增多,致管壁胶原化和纤维化。

In every layer of aneurysm wall were seen collagenic fiber, but mostly located in the middle membrane, arranged uniformly.

血管外膜未见有网状纤维。动脉壁各层均见有胶原纤维,但主要位于中膜,均匀紧密排列。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均,全层或局部区域显著变薄向外膨出,内皮细胞空泡变性或坏死脱落,部分内皮细胞剥离并突入管腔成游离状,可见血栓形成及部分血栓机化;内弹力板变薄、消失或突然中断;在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原;部分动脉瘤壁呈均质状玻璃样变,平滑肌细胞明显减少;中膜和外膜可见大量的炎性细胞浸润;瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞,胞浆内充满脂类物质及胆固醇结晶;部分动脉瘤腔内充满血栓,有的血拴已经纤维化,血栓基部机化,血栓表面有红细胞和纤维素。

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