原位

In situ hybridization was used to localize IL-4, IL-6, IL-1β, IL-2, IFN-γ and TNF-α mRNAs in abomasum of the negative control group, challenged control group, vaccinated-challenged group and vaccinated-unchallenged group goats immunized with rHco-GAL-f/m, and the numbers of cytokine mRNA(superscript +) cells in each group were also analyzed.

用rHco-GAL-f/m免疫山羊，通过原位杂交法对阴性对照组、攻虫对照组、免疫攻虫组和免疫不攻虫组山羊皱胃中白介素－1β(IL-1β)、白介素－2(IL-2)、白介素－4(IL-4)、白介素－6(IL-6)、干扰素－γ和肿瘤坏死因子－αmRNA转录进行定位，并对上述细胞因子阳性反应细胞数量进行分析。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

In conclusion, silkworm vasa plays an important role in the determination of the germ line.Whats more, the optimization of ISH has been tested.

此外，在进行原位杂交的研究中，我们探索和建立了家蚕胚胎原位杂交的方法，并对其中关键的影响因素进行了优化。

The combinated utilization of ICH and ISH can greatly improve the detective efficiency of micro-metastasis in SLNs. The result detected by ISH arere, The ISH is more sensitive than the ICH method for the detection of micromeastasis.

通过免疫组化法和原位杂交法的联合使用，可明显提高乳腺癌SLN微转移的检出率，同时也证明原位杂交法是可靠的，比免疫组化更为敏感的技术。

Subsequent surgical excisions were available in 20/33 pure FEA and in 10/11 FEA+ADH: of the 20 patients with pure FEA on CNB, none had either ductal carcinoma in situ or invasive carcinoma in their excisional biopsy, whereas 3/10 (30%) FEA+ADH on CNB showed, at subsequent surgery, more advanced lesions (2 ductal carcinoma in situ, 1 invasive carcinoma).

我们得到了20/33例单纯性FEA和10/11例FEA+ADH随后的大体手术切除资料：20例单纯性FEA大体切除标本中，无一例发现有导管原位癌或浸润性癌；而那10例FEA+ADH的大体标本中有3例（30%）发现高级病变（2例导管原位癌、1例浸润性癌）。

Methods Twenty domestic pigs of both sexes, splited through Taira line protected the portal vein and hepatic vein of orthotopic liver transplantantion.

采用改良的原位肝脏移植20例，对供肝沿Taira线劈离，对断面修整后，分别行原位移植，观察血流动力学及血液生化和温度的变化。

In control group, obturator nerve controlling right gracilis was cut off. In in situ implantation group, the right gracilis was cut off and replanted to its original site, and the obturator nerve was implanted to the muscle. In ectopic transplantation group, the right gracilis was cut off and transplanted to the muscle of the left leg, and the obturator nerve was implanted to the muscle.

对照组：切断支配右侧股薄肌的闭孔神经，造成肌肉失神经支配；原位再植组：切取右侧股薄肌，再回植于原位，闭孔神经植入肌肉；异位移植寄养组：切取右侧股薄肌，移植寄养于左侧股部，闭孔神经植入肌肉。

The humidity resistance and the sensitivity to chemical warfare agents simulants of the tuned films were also investigated.Self-assembled LBL polyaniline films were prepared from aniline monomers by in situ polymerization and the mechanism of film formation was discussed. It was proposed that the self-assembly of polyaniline occurred through the quick absorption of aniline monomer cations or aniline monomer cation radicals on the glass surfaces by electrostatic attractions to form homogeneous polymerization centers, then increased gradually by the way of upright to the glass supports for extensity, leading to the polyaniline chain growth. The polyaniline was doped under acidic condition to absorb anionic polyelectrolyte, such as sodium polystyrene sulfonate based on the electrostatic force, or to adsorb polymer based on hydrogen-bonding interactions, for example Polyvinyl Alcohol, and then recycled to fabricate_n or_n self-assembled multilayer films.

跟踪了原位聚合的具有纳米结构的聚苯胺多层自组装膜的成膜过程，提出了该自组装膜的成膜机理：聚合反应初始阶段的苯胺阳离子或苯胺阳离子自由基通过静电作用快速吸附到带负电荷的基片表面，形成均匀的聚合中心，然后以垂直于基片的方式进行链增长生成PANI；PANI在酸性条件下经现场掺杂后带正电荷，再通过静电作用吸附带负电荷的聚电解质如聚苯乙烯磺酸钠，或通过氢键作用吸附聚合物如聚乙烯醇，这样循环LBL-SA即得到_n或_n等原位聚合聚苯胺自组装膜。

Cobalt phthalocyanine was synthesized and self-assembled on the surface of nanoscale tin dioxide (SnO2) by in-situ process, marked as i, and Co-O interaction was verified to conjugate axially between macromolecule and SnO2 in CoPc/SnO2.

报道了酞菁钴分子原位自组装于纳米 SnO2 颗粒表面， CoPc 大环分子与 SnO2 表面形成 Co—O轴向相互作用，测定了原位合成方法制备的 CoPc/SnO2与浸渍法制备 CoPc/SnO2间的结合特性，并进行了可见光光催化表征及 CoPc 敏化机理探讨。

It is demonstrated that, in one aspect, in-situ zeolite has more effective catalytic capability based on unit active site, in another aspect, it has excellent ring-open activity as so called in-situ type hydrocracking catalyst can produce tail oil with generally 2-3 unit lower than traditional hydrocracking catalyst on BMCI index.

实验结果表明，原位分子筛材料不仅表现了具有更高本征催化效率的特点，而且突出表现了优异的开环裂解性能，所涉及的所谓原位型加氢裂化催化剂能获得比常规催化剂低2～3个单位的低BMCI值尾油。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。