原位

Methods Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis.

体外分离培养Ast，利用划伤的方法，建立Ast对损伤反应的实验模型，通过形态学观察、RT-PCR、免疫细胞化学、原位杂交及图像分析等方法，研究体外Ast对损伤的反应及规律。

The expression of GFAP mRNA reduced,the reactive gliosis characteristic of injured astrocyte disappeared.

PLBskG感染划伤Ast 3d后原位杂交：Ast表达GFAP mRNA水平下降，反应性胶质化的典型特征消失×200

Objective To evaluate the effects of glomerular cell proliferation and apoptosis in experimental glomerulosclerosis.

用RT-PCR方法和免疫组化法检测增殖细胞核抗原的表达，原位检测肾小球细胞凋亡指数。

An in situ injectable thermosensitive hydrogel constitutes of CS, HTCC withα,β-glycerophosphate was synthesized.

以CS及HTCC配合α，β-甘油磷酸制备原位可注射物理交联温敏水凝胶(CS/HTCC-GP水凝胶)。其溶胶-凝胶的转变时间可被控制在25°C以上。

Methods Wistar rats of 6 months old were randomly divided into normal control groupand IR group. Each group had eight rats. Euglycemic insulin clamp technique was used to determine IR and glycose infusion rate was used to IR. Cardiocyte apoptosis was evaluated by TUNEL. Heart weight and body weight were measured to calculate HW/BW. Fine structure of heart and ultramicrostructure of cardiocyte were observed.

6月龄Wistar大鼠，分为正常对照组和IR组，正常血糖胰岛素钳夹技术测定各组大鼠IR，葡萄糖输注速率表示IR情况；测量大鼠心脏重量和体重并计算心脏重量与体重之比；原位末端酶标记法检测各组大鼠心肌细胞凋亡情况；观察心脏微细结构和心肌细胞超微结构；免疫组化检测心脏间质Ⅰ、Ⅲ型胶原水平。

The studies on the compositions, origins and elastic properties of the southern Tibetan lower crust include the following 4 aspects: the petrological and geochemical festures and ages of granulitic facie rocks located in Higher Himalayas; dehydration melting experiments on the biotite-plagioclase gniess collected from Higher Himalayas at high temperature and high pressure to model the formation of the granulites; measurements of wave velocities of the lower crustal rock in situ; analysis of origin of intracrust low wave velocity layers beneath southern Tibet.

以西藏南部下地壳物质组成，成因及其弹性性质为研究目标进行了4个方面的相关研究：高喜马拉雅地区麻粒岩相岩石的岩石学，地球化学和年代学研究；麻粒岩相岩石成因的高温高压实验；原位条件下岩石的弹性波速测量；西藏高原壳内低速层的成因分析与模拟。

Result: the regions with strong to very stong labeling by the GDNF probes were as follows: the caudate-putamen, the habenular nucleuse, and some areas of the brainstem and spinal cord, the trigeminal ganglion, the dorsal root nerve ganglion.

本实验利用原位杂交技术结合RT-PCR技术观察了GDNF从E16～成年大鼠神经系统内的分布；为了更进一步研究GDNF在神经发育中的作用与作用机制，本实验还利用基因克隆技术克隆了人GDNF基因并进行了原核表达GDNF蛋白的研究。

Methods Cell cultures of8HNSCC cell lines were stained with haematoxylin and eosin,to analyze mitotic cells with anaphase bridge morphology.

用荧光结合 3 核酸探针以荧光原位杂交方法检测细胞分裂中期染色体末端端粒表达，计算平均每细胞中端粒缺失染色体末端。

Methods From 1980, 89 patients were treated with microwave heliotherapy in situ for limb salvage.

方法自 1980年以来，对 89例四肢骨肿瘤患者采用肿瘤局部切除加术中微波原位热疗进行保肢手术，男 52例，女 37例；年龄 10～ 46岁，中位年龄 18.9岁。

Objective Microwave heliotherapy was applied on the tumor-bearing bone in situ of the extremities, in order to inactivate the tumor cells for limb salvage.

目的采用微波对肿瘤瘤段骨进行原位热疗，灭活其中的肿瘤细胞，避免骨肿瘤切除后所造成的骨缺损。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。