原位

To investigate the possibility of such a transition, we studied 17 cases of MGA or atypical MGA some of which had given rise to carcinoma in situ and/or invasive ductal carcinoma using the reticulin stain, immunohistochemistry (S-100, p63, Ki-67, and p53), and a molecular approach involving microdissection and high-resolution comparative genomic hybridization and MYC chromogenic in situ hybridization.

为了研究这种转变的可能性，我们分析了17例典型或非典型的MGA，其中一些已经发展成为原位癌和/或浸润性导管癌，所用的方法是网状染色、免疫组化（S-100， p63， Ki-67， and p53）和分子技术，分子技术包括显微切割和高分辨率比较基因组杂交及MYC显色的原位杂交。

Two multifunctional agent, glycidyl methacrylate and refined ricinoleic acid , were adopted to modify EPDM in situ during compounding and vulcanization process. The effects of the modifiers on vulcanization behavior, processiability, different properties of the vulcanizates were investigated.

为此，选择了含有极性基团的多官能团小分子改性剂甲基丙烯酸缩水甘油酯和精制蓖麻油酸，在橡胶混炼和硫化过程中对EPDM进行原位改性，研究了原位反应技术对过氧化物硫化EPDM橡胶的反应特性、加工性能、力学性能、热性能、耐老化性能、耐屈挠疲劳性能及与金属的粘合强度等性能的影响，并探讨了其改性的机理。

Serologic techniques have been used in an attempt to establish diagnosis in early stages of infection.

原位杂交则不需要DNA的提取，可以在组织标本原位中显示病原菌的存在。

Due to the key challenge for complicated techniques and preparation, there were few reports about ternary systems. A series of polyimide/SiO_2-TiO_2 hybrid films with different contents of inorganic particle were fabricated using tetraethoxyl silicane and tetrabutyl titanate as precursor in situ with sol-gel method and both phases in situ. Chemical structure and micro-morphology of hybrid films were studied by means of IR, SEM and XRD.

本文以正硅酸乙酯和钛酸四丁酯为前驱体，采用原位溶胶-凝胶法和两相原位同步法制备了一系列不同含量的聚酰亚胺/SiO_2-TiO_2杂化薄膜，利用红外光谱、扫描电镜和XRD观察了薄膜的化学结构和微观结构，并测试了薄膜的热稳定性、电击穿场强和耐电晕性。

Methods: The method of immunohistochemistry was used to study the expression of CD44v6 protein in the cases, including 10 normal cervix, 15 dysplasia, 16 squamocellular carcinoma in situ , 1 adenocarcinoma in situ , 27 invasive squamocellular carcinoma, and 15 invasive adenocarcinom...

用免疫组化法检测 10例正常宫颈上皮、15例宫颈上皮不典型增生、16例宫颈原位鳞癌、1例宫颈原位腺癌、2 7例宫颈浸润鳞癌、15例宫颈浸润腺癌中CD44v6蛋白的表达，实验结果以图像处理与分析系统进行半定量处理后进行统计分析。

Methods On the basis of the previous two-cuff-technique, 120 cases of rat orthotopic liver transplantation were performed with modification of fast resecting the donor liver, two-stitch suturing suprahepatic inferior vena cavum and stoping bleeding with hot pin and so on.

120只大鼠行原位肝移植，在原二袖套法基础上改进切取供肝方法，二针法缝合供、受体肝上下腔静脉，出血点行热止血。结果大鼠原位肝移植手术时间缩短，手术成功率96.7%，3周存活率94.2%。

ATH surface was chemically bonded in sequence by tolylene diisocyanate, alkyl phenolic-formaldehyde resin and acrylonitrile-butadiene rubber.

采用表面原位化学组合改性的方法，在Al3表面顺序经原位化学反应结合上甲苯二异氰酸酯、烷基酚醛树脂、丁腈橡胶等界面改性剂。

TURNEL staining and in situ hybridization with image analysis system were applied to detect vaginal epithelial cell apoptosis and Bcl2mRNA.

采用原位末端标记法及原位杂交方法检测阴道上皮细胞凋亡及Bcl-2 mRNA，并应用图像分析系统进行测定。

The therapeutic effect of HYJDT on glomerulosclerosis was observed by way of five aspects:(1) Effect on renal tissue morphology;(2) Effect on urine protein and blood creatinine and blood uria nitrogen ;(3) Effect on superoxide dismutase and malonydialdehyde in reual cortex;(4) Effect on matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-l (TIMP-1) in glomerulus;(5) Effect on MMP-9 mRNA and TIMP-lmRNA and type-1 plasminogen activator inhibitor (PAI-1) mRAN in glomerulus.

用药治疗12周取肾，HE、PAS染色，在光镜下观察肾组织形态学变化，并在透射电镜下观察肾小球超微结构的变化。用半自动生化分析仪检测肾皮质匀浆中SOD活力、MDA含量。用免疫组化技术检测肾小球中MMP-9、TIMP-1的蛋白表达，用原位杂交技术检测MMP-9mRNA、TIMP-lmRNA、PAI-lmRNA的基因表达。免疫组化和原位杂交结果，采用计算机图象分析系统测量各自的平均吸光度。所有的实验数据均用SAS6.12统计软件包进行统计学处理。

In conclusion, silkworm vasa plays an important role in the determination of the germ line. What's more, the optimization of ISH has been tested.

此外，在进行原位杂交的研究中，我们探索和建立了家蚕胚胎原位杂交的方法，并对其中关键的影响因素进行了优化。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。