原位

Up to now, four types of cordaitean cuticles in situ and three types of dispersed ones have been reported from the Cathaysian Flora in China.

到目前为止，中国华夏植物区总共描述了 4种科达植物原位角质层和 3种分散角质层。

In the present study,in order to explore the roles of estrogen and progestin in epilepsy and elucidate their mechanisms of action, we utilized animal seizure models induced by intracerebroventricular coriaria lactone and intraperitoneal bemegride, studied the effects of estrogen and progestin on central nervous system functions in behavior,electrophysiological,cellular,molecular and gene levels by means of neuroelectrophysiology, flow cytometry, high performance liquid chromatography, immunocytochemical and in situ hybridization techniques.

为了探索雌、孕激素在癫痫发病中的作用，阐明其作用机制，本工作分别以马桑内酯侧脑室注射致痫、贝美格（Be腹腔注射致痫大鼠为实验对象，采用神经贫血电生理、流式细胞美白免疫荧光、高效液相色谱、美白免疫细胞化学、原位杂交早泻技术，从整体、行为、细胞、分子以及基因水平研究发现了雌、孕激素对大鼠中枢神经贫血系统功能的影响。

Methods: Integrated optical density was measured by ABC immuno-histochemical and in situ hybridization technique with computerized image processing. The rat′s brain was coronally sectioned after combination of EA and MEL.

采用ABC免疫组织化学和原位杂交组织化学技术，结合计算机图像处理，测定其积分光密度值。

Methods Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm×3 mm×4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with α1cDNA probe.

采取成年雄性SD大鼠肋软骨细胞，以3 mm×3 mm×4 mm大小的块状多孔HA为载体培养软骨细胞；培养3天和7天后通过扫描电镜观察HA表面和中矢横断面孔隙中有无细胞生长，同时将培养2天的细胞爬片以α1cDNA片段为探针进行原位杂交，求证培养的细胞是否软骨细胞。

The different explants, hypocotyl, terminal bud, cotyledonary node, seedling were studied to induce bud or callus with different concentrations of 6-BA and to establish a better system for transformation.

利用大豆不同品种的下胚轴、顶芽、子叶节、原位再生等几种外植体进行诱导植株再生的实验，并通过添加不同浓度的6-BA来观察诱导愈伤组织和出芽的情况，为下一步的转化实验建立一个良好的组培平台。

RESULTS: Primarily cultured cells were colored with CD44 in-situ hybridization, kytoplasm as buffy and nucleus as blue by counterstain.

结果：原代细胞以CD44原位杂交显色，胞浆呈棕黄色，胞核经苏木精复染呈蓝色。

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

ConclusionThe expression of α-B crystallin in IDC is significantly higher than that in DCIS and is positively related to lymph node metastasis. There are also higher levels of expression in the HER2+ and the triple-negative groups. This suggests that α-B crystallin is involved in the process of progression and metastasis of breast cancer.

结论α-B crystallin在乳腺浸润性导管癌中的表达明显高于导管原位癌，与淋巴结转移呈正相关，且在生物学行为更为恶性的HER2+组和三阴性组具有更高的表达水平，提示α-B crystallin蛋白可能参与了乳腺癌的浸润与转移过程。

In situ Raman spectra of cycloheptane have been characterized under high pressure generated by diamond anvil cell at room temperature.

利用金刚石对顶砧装置产生高压，在室温下对环庚烷进行了高压原位拉曼光谱研究。

Compared with the double bond of cyclopentene ring, the double bond of the norbornene ring has much more lower activation energy in the hydrogenation and the main intermediate product is 14,15-DHTCPD.

非晶态合金催化剂晶化过程的原位分析结果表明，在热处理温度低于150°C条件下其结构基本保持稳定，而高于450°C条件下热处理后晶化度大于90%，600°C以上热处理后完全晶化。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。