原位

The in-situ process of alumina particles and niobium aluminide in aluminum matrix composite, using Al-20wt% Nb2O5 system, has been studied by means of differential thermal analysis together with X-ray diffraction analysis.

利用差分扫描热分析研究了利用Al－Nb2O5系统制备Al2O3颗粒增强金属基复合材料这的原位反应过程：用X－射线衍射分析了反应过程中的相变。

The results indicate that the Ca and P ions are incorporated into the anodized alumium oxide during the anodization process, and the incorporated Ca and P are reacted to be calcium phosphate after hydrothermal treatment. The calcium phosphate grows from the holes of AAO and covers the surface of AAO layer.

结果表明：在阳极氧化过程中，钙、磷元素嵌入阳极氧化铝膜，并经水热处理反应原位生成钙磷陶瓷；钙磷陶瓷晶体从Al2O3孔洞长出并覆盖于多孔氧化膜的表面；最终获得的钙磷生物陶瓷/多孔Al2O3复合涂层具有纳米网状、多孔的结构特征。

Abstract］ Objective To identify the reasons why ametropic patients did not undergo laser in situ keratomileusis after they had received a standard pre-operative examination.

目的 分析屈光不正患者已接受准分子激光原位角膜磨镶术（laser in situ keratomileusis，LASIK）术前常规检查后最终未实施手术治疗的原因。

In the present study, influences of 6-BA (6-benzyl aminopurine) on cluster root formation, exudation of citrate and malate and their concentration in the root clusters of P-deficient white lupin plants were studied by using of non-destructive localized collection method and high-performance liquid chromatography analyze technique.

本文研究了6-BA（6-苄基腺嘌呤）处理对缺磷的白羽扇豆排根形成的影响，并利用非破坏性原位收集方法和高效液相色谱技术研究了6-BA处理对根簇分泌柠檬酸和苹果酸及组织中这两种有机酸含量的影响。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

AKP in twenty-four kinds of tissues and organs of Andrias davidianus were analyzed by the renatured electrophoresis.

用酶的原位复性电泳方法分析了中国大鲵二十四种组织器官的碱性磷酸酶。

Andrias davidianus ; alkaline phosphatase ; tissues and organs ; renatured electrophoresis

中国大鲵；碱性磷酸酶；酶原位复性电泳；组织器官

The tissue samples of small intestine, liver and blood samples from peripheral and portal vein of 4 macaques of 6-month fetus, 2-day neonate, 45-day neonate and adult were obtained after anesthetization.

通过手术途径获得胚胎6月、新生2 d、新生45 d和成年猕猴的回肠、肝脏、门静脉和外周血等标本，应用放射免疫分析法测定各标本中的VIP含量；通过免疫组化方法观察VIP在肠、肝组织内的分布；利用原位杂交法检测VIP受体1（VIP receptor 1，VIPR1）的表达。

Angiogenesie and vasculogenesis comprise the mechanisms that responsible for the development of new blood vessels. Vasculogenesis is the process of in situ formation of blood vessels from endothelial progenitor cells or angioblast. Angiogenesis involves extension of the already formed primitive vasculature by the sprouting of new capillaries through migration and proliferation of previously differentiated ECs.

新血管形成的机制有血管生成和血管新生，前者是血管内皮前体细胞或成血管母细胞在原位分化为内皮细胞并形成血管的过程，后者则是已经分化的成熟内皮细胞移行并增生使原有血管延伸。

Apoptosis of neuron in arcuate nucleus of hypothalamus was determined by TUNEL technology. To observe the effect of GNK on deterring aging process.

采用3'末端原位标记法检测下丘脑弓状核神经元凋亡的情况，探讨复方更年康作用于神经系统延缓老年雌鼠衰老的机制。3。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。