原位

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established.

分离培养正常成年大鼠肺泡Ⅱ型细胞，建立大鼠矽肺模型，用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF BmRNA和蛋白的表达。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established. In situ hybridization and irnmunohistochemieal staining were applied to detect the gene expression of TGFβ1 and PDGF-B in the alveolar type Ⅱ cells of primary culture and silicotic tissue.

分离培养正常成年大鼠肺泡Ⅱ型细胞，建立大鼠矽肺模型，用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF-B mRNA和蛋白的表达。

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞，接种并预培养 1 。5 h后，用含不同浓度 HGF的培养基继续培养至 48h，MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

Methods The expression of MMP9 protein was tested by using immunohistochemical SP method in 18 cases of normal cervical epithelium, 20 cases of carcinoma in situs of cervix, and 55 cases of invasive cervical cancer.

采用免疫组织化学SP法检测MMP9蛋白在18例正常宫颈上皮、20例宫颈原位癌、55例宫颈浸润癌中的表达。

Methods: The expression of apoptosis and the positive expression of p53, bcl－ 2 proteins were observed in 31 cases of breast cancer in situs, 20 breast dysplasia and 20 breast hyperplasia by using DNA nick end labeling technique and immunohistochemical staining . Eight normal breast tissues were used as control.

利用DNA缺口末端标记技术和免疫组织化学染色，原位观察31例乳腺癌，20例乳腺不典型增生和20例乳腺单纯性增生中细胞凋亡和p53、bcl－2蛋白的表达，以 8例正常乳腺组织作为对照。

Objective To evaluate the effect and safety of laser in situs keratomileusison low,moderate and high myopia using a scanning spot excimer laser.

目的 探讨小光斑飞点扫描准分子激光系统应用于准分子激光角膜原位磨镶术治疗低、中、高度近视的效果。

In situs hybridization histochemical technique is applied to detect the expression of five subtype of human somatostatin receptor Mrna (hSSTR1-5), including the expression model of 54 cases of various tumor specimen and the expression density of 24 lung cancer cases.

本研究采用原位杂交组织化学技术，探测高度表达人生长激素抑制素受体的5种亚型(hSSTR1-5)的54例肿瘤标本，包括54例肿瘤SSTR亚型的表达模式及其中24例肺癌的表达密度。

Methods By immunohistochemical method, the expression of bcl-2 and bad gene were observed in 8 cases of normal breast tissues 19 cases of breast hyerplasia, 20 cases of breast atypical, 48 cases of breast cancer in situs ,and test the expression of ER , PR and bcl-2 , bad in the breast cancer, Eight normal breast tissues were used control.

利用免疫组织化学染色SABC法，原位观察19例乳腺单纯性增生，20例乳腺非典型增生，48例乳腺癌组织中bcl-2、bad蛋白的表达以8例正常乳腺组织作对照。

The contact mode AFM could be used to "sweep" the slipcover and expose the mark. As an example, in situ observation of the bovine serum albumin adsorption film on highly ordered pyrolytic graphite was described. When the marks, atomic steps on HOPG, were covered by BSA molecules, contact mode AFM could "sweep" the BSA molecules away.

以对高序热解石墨（Highly ordered pyrolytic graphite，HOPG）表面牛血清白蛋白（Bovine serum albumin，BSA）吸附的原位观察为例，在BSA膜覆盖HOPG表面的原子台阶后，采用接触模式AFM扫描，将BSA"扫"开，露出HOPG原子台阶作为标记，对图像上的结构进行精确定位。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。