原位

The structure image of polymers were observed by microscopy and scanning electron micrograph. Porogenic solvent mixture was of advantages in the process of forming porous structure polymers during its polymerization. Absorption charateristic of conventional SPE column was proved by catalytic-kinetic spectrophotometric determination of nitrite, which was a system of nitritre-potassium bromate -bromothymol bule.

通过SEM表征了原位聚合法制备的SPE微柱具有多孔网状结构，显微观察法与SEM表征法结合起来，证实在有致孔剂存在的条件下有利于原位聚合反应形成多孔网状结构的SPE微柱。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

Results For the first primary lesions,most of them were stage IIa, including 12 invasive ductal caicinoma, 5DCIS, 2 invasive lobular caicinoma, a invasive carcinoma with cribriform pattern and 1 case mixed-cell carcinoma. On FFDM, 5 had microcalcification,13 were mass, 1 showed focal high attenuation and 2 were not detected. For the second primary lesions, most of them were stage 0, including 7 invasive ductal carcinoma, 12 DCIS ,1 invasive lobular carcinoma, 1 mixed-cell carcinoma.

结果；第一原发癌以Ⅱa期为主，浸润性导管癌12例，导管原位癌5例，浸润性小叶癌2例，浸润性筛状癌1例，混合细胞癌1例；5例以微钙化为主要表现，13例肿块，局部密度曾浓1例，2例未见异常；第二原发性癌以0期为主，浸润性异常癌7例，导管原位癌12例，浸润性小叶癌1例，混合细胞癌1例；11例以微钙化为主要表现，肿块3例，结构不良4例，局部密度曾浓1例，2例未见异常。

Fluorescence in situ hybridization and double FISH experiments were carried out to ascertain the chromosomal distribution patterns of the 45S and 5S ribosomal DNAs in the three species of Cucurbitaceae.

首次利用荧光原位杂交和双色荧光原位杂交技术对45S和5S rDNA在南瓜、丝瓜、冬瓜的有丝分裂中期染色体上进行了物理定位分析。

Theenvironmental risk of leachate from landfill with different operation modes depend ontheir accumulation amount rather than their sudden concentrations and fraction.(7)The high SRB metabolism activity and the accumulation of metabolitewere the main reasons of in-situ precipitation phenomenon of Cu~(2+) and Zn~(2+) inlandfill refuse in bioreactor landfill. Heavy metals in bioreactor landfill havemechanism of in-situ immobilization controlling by SRB metabolism. Thepredominant SRB in landfill refuse belongs to Desulfovibrio vulgaris sp.

7生物反应器填埋场中较高的SRB代谢活性及其逐步累积的代谢产物硫化物是填埋垃圾中的Cu~(2+)和Zn~(2+)形成硫化物沉淀而发生重金属原位固定现象主要原因，生物反应器填埋场中重金属存在原位固定的硫酸盐还原菌调控机理，初步从形态判断填埋垃圾中的优势硫酸盐还原菌属普通脱硫弧菌属Desulfovibriovulgaris sp。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定，确认Line15是一个二体异附加系，附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关，并确定了Line15的外源染色体C-分带特征带型，为目后鉴定Line15附加系的真伪提供了简便快捷的途径；对抗旱附加系Line17进行了荧光原位杂交和SSR检测，得出Line17也是一个二体异附加系，外源染色体与小麦的第四同源群短臂密切相关。

The novel restorer line Nsa restorer-1 and its progenies were analysed by genomic in situ hybridization combined with double-colour fluorescence in situ hybridization technologies, the results showed that Nsa restorer-1 was Brassica napus-Xinjiang wild Sinapis arvensis disomic alien addition line,consisting of the whole genome of Brassica napus and a homologous chromosome pair of Xinjiang wild Sinapis arvensis.

用基因组原位杂交(genomic in situ hybridization， GISH)结合双色荧光原位杂交(double-colour fluorescence in situ hybridization， dc-FISH)技术，对甘蓝型油菜新型恢复系Nsa恢1及其后代进行了研究，结果表明，Nsa恢1为甘蓝型油菜-新疆野生油菜二体异附加系，包含了甘蓝型油菜全基因组和新疆野生油菜1对染色体，这1对新疆野生油菜染色体为同源染色体。

The thermosensitive drug-loading to PNIP/AAm nanogel and drug release profile of in situ gelable PNIP/AAm nanogel dispersion were studied.

3研究了PNIP/AAm纳米凝胶粒对药物的温敏性负载及可原位凝胶化的纳米凝胶分散体原位凝胶对药物的释放行为。

In the GISH, a given genomic DNA was hybridized to chromosomes of their own or other species and signals of repetitive DNA sequences were exclusively detected.

一种新的通过基因组原位杂交进行的分子显带方法，即利用一个物种的基因组DNA作探针，对该物种或其它物种的染色体进行原位杂交，其杂交信号的检出程序是专门用于检出重复DNA顺序的。

The methods used for collecting samples and establishing specific HAB cultures are described in detail. Methods of SEM and ESEM (environmental scanning electron microscope) for Gymnodinium were developed an...

建立了赤潮监测中样品采集处理、分离和纯化培养的技术体系；建立和优化了裸甲藻扫描电镜观察技术和环境样品的扫描电镜观察方法；提出了库尔特计数是相对简便、快捷、准确、可行的细胞计数方法；应用基于反相高效液相色谱的光合色素分析技术及原位荧光激发光谱测定方法，得出不同类群浮游植物对不同的营养盐限制存在不同响应机制的结论；用荧光强度来监测浮游植物生理状态及营养状态变化具有一定的可行性，HPLC和原位荧光两种方法指示浮游植物的生理状态存在一定差异。2。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。