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Results: the positive cells mostly included ependymal cells of myelocoele, glial cells, anterior horn motor neurons, and epithelial cells of spinal pia mater and their expressions were mainly located on cell membrane. interestingly, aqp4 distribution in glial cells and ependymal cells showed certain directivity, but the former was mainly in the membrane side contacting with capillary endothelial cells and the latter mostly in the basolateral membrane of ependymal cells.

结果: 免疫组织化学染色和原位杂交结果显示胶质细胞、前角运动神经元、中央管室管膜细胞、软脊膜上皮细胞均表达阳性,aqp4主要分布于细胞膜,其中,胶质细胞和中央管室管膜细胞表达存在极性,前者主要分布在与毛细血管内皮细胞接触的一侧,后者主要分布在中央管室管膜细胞的基底侧膜。

By field-testing methods, such as static cone penetration test, field vane shear test, and pore water piezometer test, the variation of strength and the pore water pressure are measured at real time, and the marine hydrodynamics' impaction on the consolidation process of the sediments in the Yellow River Estuary was studied. It is shown that the self-consolidated sediments have a very fast consolidation rate, under self-consolidation, in 72h, the consolidation degree reaches 37% and about 142h the pore water pressure disperse over, while under the hydrodynamics, in 72h the consolidation degree reaches 52% and about 195h the pore water pressure disperse over.

在黄河三角洲刁口叶瓣潮坪上,开挖2m×1m×1m的试坑,现场取土配置黄河口快速沉积形成的流体状堆积物,以铁板部分覆盖试坑隔绝波浪和潮流作用,利用微型贯入、静力触探、十字板剪切试验、孔隙水压力监测等原位测试手段,实时测定快速沉积的海床土强度发展以及孔隙水压力消散情况,研究快速沉积的粉质海床土分别在自重条件下以及在波浪和潮波等水动力作用下的固结过程。

As long as we pull gently with tweezers pipefish is, in situ and then back on it.

我们只要用尖嘴镊子轻轻拔正,再按原位装回就可以了。

The results proved the validity of in situ pistil transformation and correct expression of foreign gene.

正反交结果显示:除草剂抗性表型在供试株系杂种中主要按1:1的比例分离,表明雌蕊原位转化获得的转基因油菜没有细胞质遗传特点。

A series of Sn/C composites was prepared by thermal condensation of coal tar pitch and tributyltin chloride with different proportions.

以煤焦油沥青为碳源、三丁基氯化锡为锡源,采用压力下聚合手段原位合成出Sn/C复合材料,并对它的电化学性能进行了研究。

Keywords Plastrum testudinis;Mesenchymal stem cells;Bone morphogenetic protein 4;In situ hybridization;Rat

龟板; 骨髓间充质干细胞;骨形态发生蛋白4;原位杂交;大鼠

Lung function were studied with animal plethysmography when suspension ended.

采用原位细胞凋亡检测技术检测肺组织的细胞凋亡情况。

Whole chromosome painting probe ; first polar body ; fluorescence in situ hybridization ; oocyte

全染色体涂抹探针;第一极体;荧光原位杂交;卵母细胞

The corrosion resistance of nanocrystalline nickel wascompared with that of Watt nickel and polycrystalline nickel plate in thesolution of NaCl and HCl by the methods of static soak and polar anodicpolarization curve and the mechanism of corrosion was analyzed. Themechanical property of nano- crystalline nickel was evaluated by macroscopictension and the microscopic process of deformation and fracture was observedby in-situ tension test under TEM.

在此基础上,采用差热分析、XRD 及 TEM 等手段研究纳米晶体镍的热稳定性;利用静态浸泡、阳极极化曲线等方法研究纳米晶体镍的耐蚀性能;采用宏观拉伸的方法考察了纳米晶体镍的力学性能,并用 TEM 原位拉伸法观察纳米晶体镍形变与断裂过程的微观过程。

Recently, it is proved that SP is also involved in pain modulation, acupuncture analgesia and immunoin- hibition. However, the role of CGRP in them is unclear Therefore, the dy- namic changes of CGRP-, SP-like immunoreactivity and expression ofα- CGRP,β-CGRP mRNAs in dorsal root ganglion and spinal cord in the arthritic rats were observed by using immunohistochemistry and in situ hybridization techniques combined with behavioral tests, respectively. Subsequently, the effects of this peoptide on the develoment of polyarthritis were investigated by intrthecal injections of CGRP and its antagonist in the rats. In addtion, GAL is a novel neuropeptide which coexists with CGRP and SP, but it may play a different role in the primary sensory system. Thus, we also observed the changes of GAL and GAL mRNA in DRG neurons and the spinal cord. The results were as follows

为此本文拟在大鼠佐剂性关节炎模型上,结合行为学测定、用免疫组织化学、原位核酸分子杂交技术观察脊髓背角及DRG中CGRP、SP、α-CGRP mRNA、β-CGRPmRNA的动态变化及电针的影响,此外,用鞘内和外周注射CGRP的工具药探讨了CGRP在佐剂性关节炎发生发展中的可能作用;甘丙肽(Galanin,GAL)也是新近发现的一个神经肽,并与CGRP和SP共存于DRG及初级传入末梢中,在神经损伤模型中脊髓CGRP、SP减少,GAL则明显增加,显示GAL与CGRP和SP的作用可能不同,因此在佐剂性关节炎模型上也同时观察了GAL和GAL mRNA的变化,实验结果如下

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。