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The vasa gene expression pattern during oogenesis of zebrafish was examined using in situ hybridization and fluorescent quantitative RT-PCR.

原位杂交结果表明:在卵母细胞生长、发育的过程中,第Ⅱ期卵母细胞的细胞质中vasa mRNA大量表达。

Several transient compounds were synthesized by the homo- and heterogeneous reactions of reactive halogen compounds, In-situ ultraviolet photoelectron spectroscopy and photoionization mass spectroscopy as well as theoretical calculations were used to study their molecular structures, stability and molecular orbital properties, their electronic structures and geometric structures were obtained for the first time; Simultaneously, the electronic structure and thermal dissociation of acetyl peroxynitrate and trifluoroacetyl peroxynitrate which have great importance in the atmospheric chemistry were studied; The substituent effects of series of halopyridines and halothiophenes were studied by the ultraviolet photoelectron spectroscopy method for the first time. The atmospheric chemical reaction between peroxyacetyl radicals with IO radicals as well as the dissociation of the most stable isomer of CH3COIO3 were calculated by density functional theory, it was found that the CH3COOIO2 isomer may be act as a new reservoir of inorganic iodine in the stratosphere.

通过活性卤素的系列均相与非均相化学反应合成了几种瞬态物种,采用原位紫外光电子能谱和光电离质谱结合量子化学理论计算对化合物的分子结构、稳定性以及分子轨道特性进行了研究,首次获得了这些物种的电子结构和几何结构的信息;同时研究了大气中有重要作用的乙酰过氧硝酸酯和三氟乙酰过氧硝酸酯的电子结构性质及热解过程;采用紫外光电子能谱手段首次研究了系列卤代吡啶和噻吩的取代基效应;通过量子化学理论计算研究了乙酰基过氧自由基与IO自由基的大气化学反应及其最稳定异构体CH3COOIO2的解离,发现该物种可能是无机碘化合物在平流层中的储库物种。

It has been found that compressive and flexural strength increases with increment of the density of lightweight alumina bubble ceramics surface-modified by aluminum dihydrogen orthophosphate and orthophorphoric acid due to in-situ formation of aluminum orthophosphate, and the results of the two surface-modifier were similar.

原位反应生成磷酸铝烧结轻质氧化铝空心球陶瓷中,磷酸二氢铝、磷酸两种结合剂结合陶瓷的耐压强度和抗折强度随配方密度提高而提高,且较为相近。

Methods: To establish the rat model of spleen-deficiency by the methods of purgation with bitter and cold nature dyugs, omproper diet and overstrain. Immunohistochemistr and in situ hybridization were used to tetect and C-fos and C-JUNmRNA expression in the hypothalamus ventral kernel, the hippocampi CA1 area and forehead cortex.

采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,免疫组化法、原位杂交法检测下丘脑腹侧核、海马CA1区、前额叶皮层C-fos水平与C-JUNmRNA表达变化及归脾汤的治疗作用。

We have developed a technique of random-primed tritium labeled DNA extension on the pachytene bivalents of rice-field eels.

采用随机引物,在黄鳝二价体上建立了氚标记引物原位DNA合成技术,并详细探索了该技术的实验条件。

Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats,but the signals were not the same on different chromosomes in intensity.

粗线期染色体荧光原位杂交结果表明,大多数染色体的末端都有端粒串联重复,但信号的强度在不同染色体上是不同的。

Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats,but the signals on different chromosomes were not the same in intensity.

粗线期染色体荧光原位杂交结果表明,大多数染色体的末端都有端粒串联重复,但信号的强度在不同染色体上是不同的。

The telomere sequences were analyzed by pachytene chromosome and extended DNA fiber FISH in rice Oryza, sativa ssp.

利用粗线期染色体和DNA纤维的荧光原位杂交技术分析了水稻广陆矮四号Oryza,sativa ssp.indica cv。

In this study, we performed sequential combined PI and DAPI staining and FISH with two different 45S rDNA clones on meiotic pachytene and mitotic metaphase chromosomes in tomato.

采用CPD染色对番茄减数分裂粗线期和有丝分裂中期染色体进行了显带分析,随后用两种不同的45S rDNA 克隆在相同的分裂相进行了荧光原位杂交定位分析。

Telomere sequences were analyzed by using pachytene chromosome and extended DNA fiber FISHin rice Oryza, sativa ssp.

利用粗线期染色体和DNA纤维的荧光原位杂交技术分析了水稻广陆矮四号Oryza sativa ssp.indica cV。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。