原位

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示，石蜡切片的鼻咽癌癌巢内，可见53BP2的强阳性表达信号，信号呈紫色颗粒状分布于胞核，核仁清晰可见；而在相同条件下，在慢性鼻咽炎组织和癌旁上皮的结缔组织，也可见弱表达信号，如淋巴细胞和平滑肌细胞；在增生的鳞状上皮细胞的核内，可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上，均无表达信号。

Finally, tissues removed from necropsy and biopsy were detected using PCR combined with species-specific probe hybrid

PCR一探针杂交在诊断的同时还可以鉴定曲霉到种。原位杂交法和PCR一探针杂交可用于石蜡包埋组织的诊断和回顾性研究。

The status of neurite regeneration was calculated and was evaluated 24 hours later.

①建立了一种原位观察神经组织块突起再生的方法，此方法简单、经济。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

The results showed that the two compounds could be separated by the nicotinamide-MIP monolithic column. The column is very useful for the enrichment and detection of nicotinamide in body fluids.

结果表明，原位聚合法制备的整体分子印迹聚合物在以纯水作流动相时对模板分子与其类似物有快速分离能力，这对于体内药物的分离富集研究具有很好的应用前景。

According to those limitations, this study would like to use the molecular biology method to enumerate the nitrobacteria in the sample fast and precisely. We use fluorescent in situ hybridization method.

基於上述原因，本研究希望以分子生物学的方法快速且精确的计数样本中硝化菌的数量，所采用的方式为原位萤光杂合法（FISH； Fluorescent In Situ Hybridization），针对铵氧化菌与亚硝酸氧化菌的16S rDNA设计一段专一性的探针。

Methods Lymphocytes were obtained from healthy donors of different ages, both sexes, and were treated with cytochalasin B. Binucleated cells were hybridized with chromosome 21 specific probe, and simultaneously, chromosome 21 loss and nondisjunction were detected.

用细胞松弛素B处理不同年龄及性别正常人外周血淋巴细胞获得的双核细胞，以21号染色体近着丝粒特异性探针进行荧光原位杂交，同时检测21号染色体丢失及不分离。

With in situ DNA hybridization, the EBV genomes have been demonstrated in the labial glands of the Sjogren′s syndrome patients (16/28), moreover the elevated content of EBV DNA has been identified in those with more severe destruction of labial glands. It is concluded that EBV, vhich has a normal site of latency at salivary glands in a nonpathogenic state, may be reactivated in Sjogren′s syndrome patients and play a role in the pathogenesis of this disease.

另外，我们用原位杂交方法观察了EB病毒DNA在干燥综合征病人唇腺组织的存在状况，结果发现28例干燥综合征病人中16例为阳性，其中腺泡组织破坏严重者，EB病毒DNA的含量亦高，并且EB病毒主要分布于大量浸润的淋巴细胞中，其次位于腺泡上皮细胞内，少量见于腺泡腔中，以上结果提示，在干燥综合征病人EB病毒处于活跃的复制状态，干燥综合征病人靶器官受损与EB病毒的活跃复制及其诱导的免疫反应密切相关。

Methods:Immunohistochemistry and nonradioactive in situ hybridization methods were used to detect the expression of p27mRNA and protein in 58 cases of osteoplastic tumors.

采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中p27蛋白和mRNA的表达状况。

We detected the expression of OCY-66, OCY-189 and OCY-142 in 28 ovarian cancers and 30 normal ovarian tissues by RT-PCR, and studied the expression of OCY-142 in these ovarian cancers and normal ovarian tissues by nonradioactive hybridization in situ.

采用RT-PCR和原位杂交的方法研究卵巢癌相关肿瘤抗原新基因在卵巢癌组织和正常卵巢组织中的表达，分析其与卵巢癌临床指标的关系。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。