原位

BACKGROUND.: Acute liver failure carries a high mortality unless urgent orthotopic liver transplantation is performed on time.

背景：急性肝功能衰竭死亡率很高，及时行急诊原位肝移植是降低死亡率的有效手段。

The result of WISH showed that the trascription of hira gene distributed widely in the early stages of embryos development and centralized to head, heart and the end of tail. Hira gene might affect the development of brain and heart. The expression characterization of colored crucian carp Cahira gene in different tissues and different stages of embryogenesis were also investigated by RT-PCR.

胚胎原位杂交显示，Hira基因的转录本在银鲫胚胎早期广泛分布，而在胚胎后期主要集中于头部、心脏及尾芽尖部，说明也对其脑部和心脏的发育有影响。

In situ hybridization histochemistry and Immunohistochemistry technique were applied to the detection of CD14 in dental tissue.

原位杂交和免疫组织化学研究提示CD14在牙髓炎、牙龈炎中有重要作用。

We detected the expression of VEGF mRNA by in situ hybridization and Ki 67 protein by immune histochemistry.

原位杂交法检测瘤组织血管内皮生长因子mRNA表达；免疫组化法检测瘤组织中细胞增殖核抗原Ki 67的表达。

(1) In our research,frequent expression of BSAP in H/RS cell of classical Hodgkins disease provides further evidence for its B-cell origin,and the expression of BSAP on H/RS cells also can be in favor of the identification of H/RS cells and can distinguish HL from ALCL.(2) Expression of BSAP is located in nucleoli of cells,while that of CD20 in membrane.

结果 (1)33例cHL中，30／33(90.91％)H／RS细胞和33／33(100％)背景淋巴细胞BSAP表达阳性，而H／RS细胞10／33(30.30％)CD20阳性，1／33(3.03％)CD45RO阳性，31／33(93.94％)CD30阳性，25／33(75.75％)CD15阳性；(2)33例HL石蜡刮片组织中有16例(48.48％)出现IgH基因重排阳性，显微切割的19管H／RS细胞有14管(73.68％)扩增阳性，而12管背景淋巴细胞有2管(16.67％)扩增阳性；(3)测序分析周新华第一军医大学病理学教研室获得了一段免疫球蛋白重链可变区的重排片断；（4）原位PCR结果发现杂交的阳性颗粒不仅出现在探针来源的同一病例也出现在不同病例的HillS细胞核上，同时在部分周围背景淋巴细胞核上也见到阳性颗粒。

Zwieniecki and Holbrook 1999, Canny et al. 2001, recording diurnal variation in conductance in situ (Zwieniecki and Holbrook 1999, Bucci et al. 2003, Domec et al. 2006), and inducing cavitation by drying plants in the laboratory Hacke and Sperry 2003, Stiller et al.

1998年， zwieniecki和霍尔布鲁克日前， 1999年，憎恨等， 2001 ），记录昼夜变化在电导原位（ zwieniecki和霍尔布鲁克日前， 1999年bucci等人， 2003年多梅克等人2006年），并诱导蚀干燥植物在实验室（ hacke和sperry 2003年， stiller等人2005年）。

The results indicate thatC particles exhibit finer size which are mostly less than 2μm and homogeneous distribution in the matrix.

研究结果表明：原位合成的C增强相颗粒细小，大部分小于2μm，在α-Fe基体中均匀分布。

Fine TiB_2 particles are produced in situ-B_4C through reaction of B_4C + 2TiC = 2TiB2+3C and B4C-based composites are prepared by hot-press process at 2050℃.

通过TiC与B4C的化学反应，在B4C基体内原位生成TiB2颗粒，获得的TiB2颗粒粒径一般在3μm以下。

Preparation and collection of specimens: To kill the rabbits at different time and collect the specimens for pathology, electron microscope, RT-PCR and orthotopic hybridism in situ.

标本留取：分别于术后第2、4周处死假手术组大白兔各2只，于术后即刻随机处死对照组和通心络组大白兔各2只，损伤后3天、1周、2周、4周和6周随机处死对照组和通心络组大白兔各4只，分别留取病理、电镜、逆转录-聚合酶链式反应和原位杂交试验标本。

Use biochemical method to observe the effect of electro-acupuncture on AchE、SOD、MAO、NO、NOS of senile dementia AD rat's brain tissue; use immunohistochemistry technique to observe protein kinase expression of hippocampal; use optical and electron microscope to observe morphosis change of rat's brain tissue; use in situ hybridism technique to observe aspartate receptor mRNA expression of hippocampal; use water and electric maze experiment to observe rat escape latent period and mistake times, to understand it's learning and memory ability.

分别用生化方法观察电针对AD大鼠脑组织胆碱酯酶、超氧化物歧化酶、单胺氧化酶、一氧化氮、一氧化氮合酶的影响；用免疫组化方法观察海马组织蛋白激酶的表达；用光、电镜方法观察大鼠脑组织形态结构的变化；用原位杂交方法观察海马组织天门冬氨酸受体rnRNA的表达；用水迷宫和电迷宫实验观察大鼠逃避潜伏期和错误次数，以了解其学习记忆能力。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。