原位

Results After articular cartilage injury, the degeneration and denature of the cartilage occurred, and the cluster expressed collagen type Ⅹ and apoptosis appeared.Conclusion Apoptosis may play a key role in the pathogenesis and prognosis of osteoarthritis.

为进一步探讨骨关节病的发病机制，我们收集了骨关节病的临床标本，采用原位杂交及原位末端标记的方法，观察Ⅹ型胶原和凋亡细胞在骨关节病关节软骨组织中的分布，并探讨凋亡与骨关节病的关系。

In the School, there is a high-level experiment research center furnished with the atomic absorption spectrophotometer , comprehensive thermal analyzer, in-situ high temperature and high resolution SEM, in-situ high temperature XRD, RAMAN, ICP, FTIR, AFM, CHN, laser particle analyzer, GPC, dynamic mechanical analyzer, laser flash thermal conductivity determinator , high temperature graphite furnace , PECVD, magnetron sputtering furnace, etc.

学院建设具有高水平的实验研究中心，拥有ICP、DSC、高温原位HRSEM、、高温原位XRD、RAMAN、激光粒度仪、GPC、CHN、DMA、孔径与比表面测定仪、激光闪光导热率测定仪、高温石墨炉、真空热压炉、PECVD、磁控溅射仪等仪器设备。

From the comparison of the flat dilatometer test and several other in-situ tests, a conclusion can be drawn that the DMT is very suitable for test parameter measurement of seaside silt.

通过对扁铲侧胀试验与其它原位试验方法的比较，发现扁铲试验能很好地确定海滩区淤泥路基原位试验参数。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

ISH and FISH were used for detection of EBER and t(8;14)respectively.

用原位杂交技术检测EBER，用荧光原位杂交检测t(8；14)。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

In test group fibroblast growth factor immunoreactivity was detected in 89% of the samples, mRNA was detected in 20% of the samples. In control group no fibroblast growth factor immunoreactivity and mRNA was detected.

结果：实验组30例免疫组化和原位杂交的阳性率分别为89％和20％，对照组6例免疫组化和原位杂交均为阴性，两组免疫组化方法检测阳性率有显著差异。

Cu-Ag-Ce in situ filamentary composite with good combination of high-strength and high-conductivity was prepared.

研究了稀土添加剂Ce、Y和Gd对Cu-Ag原位纤维复合材料结构和性能的影响，制备了具有高强度和高导电性相结合的Cu-Ag-Ce原位纤维复合材料。

Orthotopic hybridism in situ: To study the expression in situ of MMP-3 and NF-κB in frozen slices.

原位杂交：观察MMP-3和NF-κB p65在对照组和通心络组冰冻切片中的原位表达。

Combined with animal model of peradaptation to myocardium ischemia , the research observed the level of enzymatic dynamics of myocardium, SOD,MDA,ET and correlation peptide of calcitonin gene in peripheral blood and cardiac function, apoptic cell and expression of apoptosis regulatory gene, ultrastructure of myocardium with BIOPAC16, in situ end labeling and hybridism, optic microscope and electron microscope. The study of animal model of peradaptation to myocardium ischemia illustrated that the peradaptation and pretreatment with Wenxin capsule could be positively protective for the injury of ischemia reperfusion. The protection is mainly reflected by reduction of infarction area, correction of heart rhythm abnormality of ischemia reperfusion, melioration of the contraction and relaxation function of myocardium, recovery of the subcellular structure and themelioration of the abnormality of biochemical and bioactive substance in the myocardium.

并结合心肌缺血预适应动物模型，采用BIOPAC16导生理记录和分析处理系统处理心肌功能指标，采用原位末端标记及原位杂交技术标记凋亡细胞及凋亡相关调控基因的表达，采用光电镜技术观察心肌超微结构的变化，同时观察外周血心肌酶、超氧化物歧化酶、丙二醛、内皮素、降钙素基因相关肽等水平，结果表明心肌缺血预适应及温心胶囊预处理对心肌缺血再灌注损伤具有肯定的保护作用，其保护心脏作用主要表现在缩小心肌梗死范围、抗缺血再灌注心律失常、改善心肌收缩和舒张功能、减轻心肌超微结构损伤，调节心肌生化物质及生物活性物质的生成和释放。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。