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Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内,隐孢子虫出现连续发育阶段,包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊,在60~72 h内形成卵囊;用感染48 h的细胞培养上清接种于免疫抑制小鼠, 10 d后有隐孢子虫卵囊排出。

Results MRI findings of central neurocytoma are specific in 5 cases. The ganglioglioma was found in the tempora and occipital lobe in 7 cases,in the brainstem in 1 case, cystic-solid tumor was observed in 5 cases with extramural nodule in 3 cases, obviously thickened gyri were revealed in 1 cases and central necrosis in 2 cases.The 3 cases of Lhermitte-Duclos disease include the specific molecular layer imaging features.

结果 5例中枢神经细胞瘤有一定的MRI特征,3例诊断正确。8例神经节细胞胶质瘤中,7例位于或主要位于颞、枕叶,1例位于桥脑和延髓,表现为囊实性者5例,实性部分位于囊外的有3例,呈实性表现为局部脑回增厚者1例,囊变坏死明显者2例。3例小脑发育不良性节细胞瘤有特征性的条纹状或分层状表现。

Results: MR demonstrated capsule wall and papillary solid lesions in one case with cystic and solid lesions of hepatobiliary cystadenoma. Poly cystic form tan if different sine in sew case, capsule wall and septation was thick, part of capsule wall was obviously thickened and nodes form, intrahepatic bile duct was dilated, large poly-antrum cystic keen form structure in one case, capsule wall and septation were smooth, part of capsule wall was uniformly thicken, capsule wall, septation, mural nodus form and solid lesions in three cases wee all slightly hyperintense on T2WI and slightly hypointense on T1WI. Part of cyst fluid was hypointense on T2WI the diameter if tumor ranged from 3.4cm to 13.5cm. Part of capsule wall, septation, solid lesions and mural nodus west obviously enhanced in arterial phase in three cases, the extent of enhancement showed a little degrade in port vein phase, balance phase and delay phase, but dense to liver parenchyma at synchronization.

结果:3例肝内胆管囊腺瘤中1例呈囊实性结构,可见囊壁及乳头状实性肿块;1例为多个大小不等的多房囊状结构,囊壁及分隔光滑,囊壁局部明显增厚,有壁结节,周围见轻度的肝内胆管扩张;1例为巨大的多房囊状结构,囊壁及分隔光滑,局部均匀增厚;3例中的囊壁、分隔、壁结节及实性部分均呈T2WI稍高信号、T1WI稍低信号,囊内液性部分均呈T2WI高信号、T1WI低信号;肿瘤瘤体大小径线范围3.4~13.5cm.3例肝内胆管囊腺瘤中囊壁、分隔、实性肿块和壁结节动脉期均明显强化,门脉期、平衡期及延迟期强化程度略减低,强化程度均高于同期肝实质。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Results Among all 25 cases of lymphangioma,Within 20 cases of cystic lymphangioma,single cyst was showed in 14 cases and multilocular cyst was showed in 6 cases in plain scan,and little enhancement of capsule wall and septal in 8 cases in enhancement scanning. Cystic-solid mass was showed in 4 cases of cavernoma lymphangioma in plain scan,and solid part of mass was enhanced obviously in enhancement scanning.1 capillary ymphangioma showed the skin and hypodermic thicking,and part of area showed little beehive-like feature. Enhancement scanning showed slight irregularity enhancement.

结果 25例淋巴管瘤中20例囊性淋巴管瘤,CT平扫14例表现为单一囊肿,6例表现为多房性囊肿,囊腔内见有分隔;增强扫描8例出现囊壁或间隔的轻微强化。4例海绵状淋巴管瘤平扫均表现为囊实性肿块,增强检查实性部分有明显分期增强;1例毛细管型淋巴管瘤表现为局部皮肤及皮下组织的增厚,部分区域见有小蜂房样表现,增强扫描见有轻度不规则强化。

Antheridia occurred in the cushion on the dorsal and ventral surfaces of gametophytes while archegonia were observed on the ventral surface only. On hermaphroditic gametophytes, antheridia and archegonia were usually intermingled. Both types of gametangia were eusporangiate.

藏精器与藏卵器分别在孢子洒播10及12月后发生;藏精器长在中肋的背面及腹面,藏卵器只在腹面发生;两性配子体的藏精器与藏卵器混生一起,两者均属厚囊蕨型。

Resujts:① The distribution of lesions are mostly symmetrical, extensive, predominant in the middle and lower lung fields, and posterior areas in peripheral;② There are so many HRCT findings of lung, ILD is predominant: intralobular interstitial thickening 46 cases (100%), ground-glass opacity 41 cases (89.13%), peribronchovascular or centrilobular interstitial thickening 40 cases (86.96%), interlobular septal thickening 38 cases (82.61%), irregular linear opacity 37 cases (80.43%), small nodular opacity 34 cases (73.91%), subpleural line 27 cases (58.70%), bmnchiectasis or bronchiolectasis 19 cases (41.30%), patch opacity 18 cases (39.13%), expiratory mosaic sign 15 cases (32.61%), interface sign 14 cases (30.43%), honeycombing 12 cases (26.09%), emphysema or bulla 3 cases, cystic airspace suspected 1 case, and atelectasis suspected 1 case;③ Mediastinum and pleura: multiple small lymphonodi in mediastinum 41 cases (89.13%), pleural thickening or rough 38 cases (82.61%), esophagoectasis 11 cases (23.91%), unilateral little pleural fluid 1 case, and mediastinal emphysema 1 case.

结果:①皮肌炎肺部病变分布呈对称、广泛、偏中下、偏外后的特点;②肺部HRCT表现多样,以肺间质性改变为主:小叶内间质增厚46例(100%),磨玻璃影41例(89.13%),支气管血管束增宽或小叶核心增大40例(86.96%),小叶间隔增厚38例(82.61%),不规则纤维索条影37例(80.43%),结节影34例(73.91%),胸膜下线27例(58.70%),支气管或细支气管扩张19例(41.30%),斑片影18例(39.13%),呼气相马赛克征15例(32.61%),界面征14例(30.43%),蜂窝影12例(26.09%),肺气肿或肺大泡3例,单纯囊状气腔1例,肺不张l例;③纵隔及胸膜:纵隔小淋巴结影41例(89.13%),胸膜增厚或毛糙38例(82.61%),食管扩张11例(23.91%),单侧少量性胸腔积液1例,纵隔气肿1例。

The results were as follows: Compared with control group, the weight and the relative weight of thymuses, bursas of Fabricius and spleens of experiment group increased significantly(P<0.05) or extremely significantly(P<0.01). In experiment group, the cortex of thymic lobule, bursa nodule and Periarterial Lymphatic Sheaths thicken obviously; the volume of bursa nodule, splenic nodule and ellipsoid augmented, and the germinal center of splenic nodule were obvious; the thymic corpuscle increased; the plica of bursas of Fabricius developed well and the degenerating of it retarded.

结果表明:试验组鸡胸腺、腔上囊、脾的重量和器官指数均高于对照组,差异显著(P<0.05)或极显著(P<0.01);试验组的胸腺小叶皮质增厚,胸腺小体增多;腔上囊皱襞发达,腔上囊小结体积大,皮质较厚,退化延缓;脾的动脉周围淋巴鞘增厚,脾小结体积大,生发中心明显,椭球增大。

The Actinobacteria are distinguished by producing actinospres: entire cells encysted in thick walls to form resistant spores.

放线菌的特征是能够产生放线菌孢子,它是一种被囊的具有厚壁的完整细胞,具有一定的抵抗能力。

In malignant room skin tumour early phase can see something without the aid of instruments in regular or opaque dirty tier or wall have a lot of white or the gray pellet and form festival or the lamina on tier of pleurae Piece with the development of tumour change thickly long and change thickly long full pleura surface tubercle the tumour tubercle stretches room skin to all sides postpone continuing becoming movie cyst lungs uses the person The cellar is getting smaller as shrinking more and makes to be put into much trouble inclining a chest wall subside.

在恶性间皮瘤早期,肉眼可见在正常或不透明的脏层或壁层胸膜上有众多白色或灰色颗粒和结节或薄板块,随着肿瘤的发展,胸膜表面愈来愈变厚,长满结节,间皮瘤结节向四方伸延,连续成片,包囊肺脏使其窖越缩。。。在恶性间皮瘤早期,肉眼可见在正常或不透明的脏层或壁层胸膜上有众多白色或灰色颗粒和结节或薄板块,随着肿瘤的发展,胸膜表面愈来愈变厚,长满结节,间皮瘤结节向四方伸延,连续成片,包囊肺脏使其窖越缩越小

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