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This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明,酒酒球菌SD-2a原生质体制备的最佳条件为:菌龄20 h,青霉素G钠质量浓度0.5μg/mL,酶质量浓度1mg/mL,酶解时间30 min,渗透压稳定剂采用SMM缓冲液,再生培养基采用添加0.7 mol/LKCl的ATB培养基,再生培养时采用双层培养基厌氧培养,可以保证较好的形成率和再生率。

This paper analyzes the procreant gases through the intermittent anaerobic digestion experiences of MSW.

通过对城市生活垃圾高温间歇厌氧消化的实验,对其产气进行分析。

The main strains used in industry fermentation are Propionibacterium freudenrechii and Pseudomonas denitrificans. The basic characteristics, biosynthesis and fermentation of vitamin B12 are reviewed.

VB12主要由古生菌和一些真细菌通过有氧或厌氧两种途径合成,工业上主要采用费式丙酸菌Propionibacterium freudenrechii)和脱氮假单胞菌(Pseudomonas denitrificans进行发酵生产。

Methods A strain of Propionibacterium acnes from rumen fluid of health cow with permanent rumen fistula under sterile condition was isolated by segregation procedure of anaerobic bacterium and specific medium SLB, and identified by extraction of the genome DNA of isolation bacterium, clone of the 16S rRNA gene, and sequencing of the strain.

无菌采取装有瘤胃瘘奶牛的瘤胃液,按照厌氧菌分离步骤,通过丙酸生成菌株的特异性培养基SLB进行筛选,提取分离菌的基因组DNA,克隆其16S rRNA基因,进行序列测定,分离出一株痤疮丙酸杆菌。

Apparently,higher ratio of propionic/acetic acid is not conducive to the improvement of phosphorus removal.

说明在厌氧/缺氧环境下,碳源中丙酸比例的提高不利于系统中磷的去除。

In this paper,the research progress on psychrophilic methanogens was reviewed,with the focus on thei...

本文针对研究者广泛关注的热点问题,从分离培养及生理生化特性、适冷机制和分子生物学研究几个方面,对嗜冷产甲烷菌的研究进展进行了全面的综述,并对其在低温厌氧生物处理技术中的应用前景进行了分析和展望。

The performance and the sludge characteristics of an upflow anaerobic sludge blanket reactor were investigated for the treatment of low concentration (COD 400~600 mg·L-1) domestic wastewater at psychrophilic temperature (15±1℃).

考察了厌氧处理低温(15±1℃低浓度(COD 400~600 mg·L-1)模拟生活污水试验规模UASB反应器的工作状况及污泥特性。

In this paper, the research progress on psychrophilic methanogens was reviewed, with the focus on their culture, isolation, physiological and biochemical characters, molecular biological characters, and cold -adapted mechanisms. The application prospects of psychrophilic methanogens in low-temperature anaerobic technology were also analyzed.

本文针对研究者广泛关注的热点问题,从分离培养及生理生化特性、适冷机制和分子生物学研究几个方面,对嗜冷产甲烷菌的研究进展进行了全而的综述,并对其在低温厌氧生物处理技术中的应用前景进行了分析和展望。

Psychrophilic methanogens ; low-temperature anaerobic bio-treatment ; cold-adapted mechanism ; molecular biological technology

嗜冷产甲烷菌;低温厌氧生物处理;适冷机制;分子生物学技术

This paper describes existing comprehensive MSW management and bio energy development practices in EU countries, deals mainly with anaerobic fermentation technology for organic waste and puts forward suggestions for bio-energy development and putrescible organic waste management and disposal in China.

本文介绍了EU各国MSW综合管理和生物能源的现状和发展,着重介绍了有机垃圾厌氧产沼工艺、技术及其应用,提出了我国生物能源技术发展及可腐有机废物管理与处置建议。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。